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Journal of Virology, June 2001, p. 5405-5409, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5405-5409.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Integrin alpha vbeta 1 Is an Adenovirus Coreceptor†

Erguang Li, Swati L. Brown, Dwayne G. Stupack, Xose S. Puente, David A. Cheresh, and Glen R. Nemerow*

Department of Immunology, The Scripps Research Institute, La Jolla, California 92037

Received 19 January 2001/Accepted 9 March 2001

The human embryonic kidney (HEK293) cell line, commonly used for recombinant adenovirus (Ad) propagation, does not express the Ad coreceptor alpha vbeta 3 or alpha vbeta 5 integrins, yet these cells are efficiently infected by Ad vectors. Here we demonstrate that Ad binds to HEK293 cells via the fiber receptor CAR and is subsequently internalized via interaction with integrin alpha vbeta 1. Function-blocking antibodies directed against alpha v or beta 1, but not beta 3, beta 5, or alpha 5, integrin subunits block Ad infection and viral endocytosis. Therefore, alpha vbeta 1 serves as a coreceptor for Ad infection, and the lack of beta 3 and/or beta 5 but the relatively high expression of alpha vbeta 1 integrins on certain tumor cell types may explain why these cells are readily transduced by Ad vectors.

* Department of Immunology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 784-8072. Fax: (858) 784-8472. E-mail: gnemerow{at}scripps.edu.

dagger This is manuscript no. 13843-IMM from the Department of Immunology.

Journal of Virology, June 2001, p. 5405-5409, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5405-5409.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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