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Journal of Virology, June 2001, p. 5363-5369, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5363-5369.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Deletion of the Cytoplasmic Tail of the Fusion Protein of the Paramyxovirus Simian Virus 5 Affects Fusion Pore Enlargement

Rebecca Ellis Dutch1,dagger and Robert A. Lamb2,*

Department of Biochemistry, Molecular Biology and Cell Biology1 and Howard Hughes Medical Institute,2 Northwestern University, Evanston, Illinois 60208-3500

Received 12 January 2001/Accepted 1 March 2001

The fusion (F) protein of the paramxyovirus simian parainfluenza virus 5 (SV5) promotes virus-cell and cell-cell membrane fusion. Previous work had indicated that removal of the SV5 F protein cytoplasmic tail (F Tail- or FDelta 19) caused a block in fusion promotion at the hemifusion stage. Further examination has shown that although the F Tail- mutant is severely debilitated in promotion of fusion as measured by using two reporter gene assays and is debilitated in the formation of syncytia relative to the wild-type F protein, the F Tail- mutant is capable of promoting the transfer of small aqueous dyes. These data indicate that F Tail- is fully capable of promoting formation of small fusion pores. However, enlargement of fusion pores is debilitated, suggesting that either the cytoplasmic tail of the F protein plays a direct role in pore expansion or that it interacts with other components which control pore growth.

* Corresponding author. Mailing address: Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2153 N. Campus Dr., Evanston, IL 60208-3500. Phone: (847) 491-5433. Fax: (847) 491-2467. E-mail: ralamb{at}northwestern.edu.

dagger Present address: Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536-0298.

Journal of Virology, June 2001, p. 5363-5369, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5363-5369.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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