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Journal of Virology, June 2001, p. 5049-5058, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5049-5058.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Virological Properties and Nucleotide Sequences of Cas-E-Type Endogenous Ecotropic Murine Leukemia Viruses in South Asian Wild Mice, Mus musculus castaneus

Hidetoshi Ikeda,1,2,* Kanako Kato,1 Hiroshi Kitani,1 Takako Suzuki,1 Takamasa Yoshida,2,dagger Yutaka Inaguma,2,3 Naoyuki Yamamoto,4,Dagger Jun-Gyo Suh,4,§ Byung-Hwa Hyun,4,|| Takahiro Yamagata,4 Takao Namikawa,4 and Takeshi Tomita4

National Institute of Animal Health, Tsukuba, Ibaraki-ken,1 and Aichi Cancer Center Research Institute, Nagoya,2 Institute for Developmental Research, Aichi Human Service Center, Kasugai,3 and Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya,4 Aichi-ken, Japan

Received 16 November 2000/Accepted 2 March 2001

Two types of endogenous ecotropic murine leukemia viruses (MuLVs), termed AKV- and Cas-E-type MuLVs, differ in nucleotide sequence and distribution in wild mouse subspecies. In contrast to AKV-type MuLV, Cas-E-type MuLV is not carried by common laboratory mice. Wild mice of Mus musculus (M. m.) castaneus carry multiple copies of Cas-E-type endogenous MuLV, including the Fv-4r gene that is a truncated form of integrated MuLV and functions as a host's resistance gene against ecotropic MuLV infection. Our genetic cross experiments showed that only the Fv-4r gene was associated with resistance to ecotropic F-MuLV infection. Because the spontaneous expression of infectious virus was not detected in M. m. castaneus, we generated mice that did not carry the Fv-4r gene but did carry a single or a few endogenous MuLV loci. In mice not carrying the Fv-4r gene, infectious MuLVs were isolated in association with three of six Cas-E-type endogenous MuLV loci. The isolated viruses showed a weak syncytium-forming activity for XC cells, an interfering property of ecotropic MuLV, and a slight antigenic variation. Two genomic DNAs containing endogenous Cas-E-type MuLV were cloned and partially sequenced. All of the Cas-E-type endogenous MuLVs were closely related, hybrid-type viruses with an ecotropic env gene and a xenotropic long terminal repeat. Duplications and a deletion were found in a restricted region of the hypervariable proline-rich region of Env glycoprotein.

* Corresponding author. Mailing address: National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba 305-0856, Japan. Phone: 81-298-38-7859. Fax: 81-298-38-7880. E-mail: hikeda{at}niah.affrc.go.jp.

dagger Present address: Environmental Countermeasures Division, Aichi Prefecture Environment Department, Naka-ku, Nagoya, Aichi-ken 460-8501, Japan.

Dagger Present address: Hokkaido National Agricultural Experiment Station, Toyohiraku, Sapporo 062-8555, Japan.

§ Present address: Experimental Animal Center, College of Medicine, Hallym University, Chunchon, Kangwon-do 200-702, Korea.

|| Present address: Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon 305-600, Korea.

Journal of Virology, June 2001, p. 5049-5058, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5049-5058.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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