This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, P.
Right arrow Articles by Lai, M. M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, P.
Right arrow Articles by Lai, M. M. C.

 Previous Article  |  Next Article 

Journal of Virology, June 2001, p. 5009-5017, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5009-5017.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Heterogeneous Nuclear Ribonucleoprotein A1 Binds to the 3'-Untranslated Region and Mediates Potential 5'-3'-End Cross Talks of Mouse Hepatitis Virus RNA

Peiyong Huang1 and Michael M. C. Lai1,2,*

Department of Molecular Microbiology and Immunology1 and Howard Hughes Medical Institute,2 University of Southern California Keck School of Medicine, Los Angeles, California 90033-1054

Received 3 November 2000/Accepted 6 March 2001

The 3'-untranslated region (3'-UTR) of mouse hepatitis virus (MHV) RNA regulates the replication of and transcription from the viral RNA. Several host cell proteins have previously been shown to interact with this regulatory region. By immunoprecipitation of UV-cross-linked cellular proteins and in vitro binding of the recombinant protein, we have identified the major RNA-binding protein species as heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1). A strong hnRNP A1-binding site was located 90 to 170 nucleotides from the 3' end of MHV RNA, and a weak binding site was mapped at nucleotides 260 to 350 from the 3' end. These binding sites are complementary to the sites on the negative-strand RNA that bind another cellular protein, polypyrimidine tract-binding protein (PTB). Mutations that affect PTB binding to the negative strand of the 3'-UTR also inhibited hnRNP A1 binding on the positive strand, indicating a possible relationship between these two proteins. Defective-interfering RNAs containing a mutated hnRNP A1-binding site have reduced RNA transcription and replication activities. Furthermore, hnRNP A1 and PTB, both of which also bind to the complementary strands at the 5' end of MHV RNA, together mediate the formation of an RNP complex involving the 5'- and 3'-end fragments of MHV RNA in vitro. These studies suggest that hnRNP A1-PTB interactions provide a molecular mechanism for potential 5'-3' cross talks in MHV RNA, which may be important for RNA replication and transcription.

* Corresponding author. Mailing address: Howard Hughes Medical Institute, Department of Molecular Microbiology and Immunology, University of Southern California Keck School of Medicine, 2011 Zonal Ave., HMR-401, Los Angeles, CA 90033-1054. Phone: (323) 442-1748. Fax: (323) 342-9555. E-mail: michlai{at}hsc.usc.edu.

Journal of Virology, June 2001, p. 5009-5017, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.5009-5017.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Liu, P., Li, L., Keane, S. C., Yang, D., Leibowitz, J. L., Giedroc, D. P. (2009). Mouse Hepatitis Virus Stem-Loop 2 Adopts a uYNMG(U)a-Like Tetraloop Structure That Is Highly Functionally Tolerant of Base Substitutions. J. Virol. 83: 12084-12093 [Abstract] [Full Text]  
  • Zust, R., Miller, T. B., Goebel, S. J., Thiel, V., Masters, P. S. (2008). Genetic Interactions between an Essential 3' cis-Acting RNA Pseudoknot, Replicase Gene Products, and the Extreme 3' End of the Mouse Coronavirus Genome. J. Virol. 82: 1214-1228 [Abstract] [Full Text]  
  • Beerens, N., Snijder, E. J. (2007). An RNA Pseudoknot in the 3' End of the Arterivirus Genome Has a Critical Role in Regulating Viral RNA Synthesis. J. Virol. 81: 9426-9436 [Abstract] [Full Text]  
  • Goebel, S. J., Miller, T. B., Bennett, C. J., Bernard, K. A., Masters, P. S. (2007). A Hypervariable Region within the 3' cis-Acting Element of the Murine Coronavirus Genome Is Nonessential for RNA Synthesis but Affects Pathogenesis. J. Virol. 81: 1274-1287 [Abstract] [Full Text]  
  • Karakasiliotis, I., Chaudhry, Y., Roberts, L. O., Goodfellow, I. G. (2006). Feline calicivirus replication: requirement for polypyrimidine tract-binding protein is temperature-dependent.. J. Gen. Virol. 87: 3339-3347 [Abstract] [Full Text]  
  • Harris, D., Zhang, Z., Chaubey, B., Pandey, V. N. (2006). Identification of Cellular Factors Associated with the 3'-Nontranslated Region of the Hepatitis C Virus Genome. Mol. Cell. Proteomics 5: 1006-1018 [Abstract] [Full Text]  
  • Johnson, R. F., Feng, M., Liu, P., Millership, J. J., Yount, B., Baric, R. S., Leibowitz, J. L. (2005). Effect of Mutations in the Mouse Hepatitis Virus 3'(+)42 Protein Binding Element on RNA Replication. J. Virol. 79: 14570-14585 [Abstract] [Full Text]  
  • Schelle, B., Karl, N., Ludewig, B., Siddell, S. G., Thiel, V. (2005). Selective Replication of Coronavirus Genomes That Express Nucleocapsid Protein. J. Virol. 79: 6620-6630 [Abstract] [Full Text]  
  • Brunner, J. E., Nguyen, J. H. C., Roehl, H. H., Ho, T. V., Swiderek, K. M., Semler, B. L. (2005). Functional Interaction of Heterogeneous Nuclear Ribonucleoprotein C with Poliovirus RNA Synthesis Initiation Complexes. J. Virol. 79: 3254-3266 [Abstract] [Full Text]  
  • Yu, L., Markoff, L. (2005). The Topology of Bulges in the Long Stem of the Flavivirus 3' Stem-Loop Is a Major Determinant of RNA Replication Competence. J. Virol. 79: 2309-2324 [Abstract] [Full Text]  
  • Sola, I., Moreno, J. L., Zuniga, S., Alonso, S., Enjuanes, L. (2005). Role of Nucleotides Immediately Flanking the Transcription-Regulating Sequence Core in Coronavirus Subgenomic mRNA Synthesis. J. Virol. 79: 2506-2516 [Abstract] [Full Text]  
  • Choi, K. S., Mizutani, A., Lai, M. M. C. (2004). SYNCRIP, a Member of the Heterogeneous Nuclear Ribonucleoprotein Family, Is Involved in Mouse Hepatitis Virus RNA Synthesis. J. Virol. 78: 13153-13162 [Abstract] [Full Text]  
  • Christian, K., Lang, M., Maurel, P., Raffalli-Mathieu, F. (2004). Interaction of Heterogeneous Nuclear Ribonucleoprotein A1 with Cytochrome P450 2A6 mRNA: Implications for Post-Transcriptional Regulation of the CYP2A6 Gene. Mol. Pharmacol. 65: 1405-1414 [Abstract] [Full Text]  
  • Ropp, S. L., Wees, C. E. M., Fang, Y., Nelson, E. A., Rossow, K. D., Bien, M., Arndt, B., Preszler, S., Steen, P., Christopher-Hennings, J., Collins, J. E., Benfield, D. A., Faaberg, K. S. (2004). Characterization of Emerging European-Like Porcine Reproductive and Respiratory Syndrome Virus Isolates in the United States. J. Virol. 78: 3684-3703 [Abstract] [Full Text]  
  • Goebel, S. J., Hsue, B., Dombrowski, T. F., Masters, P. S. (2004). Characterization of the RNA Components of a Putative Molecular Switch in the 3' Untranslated Region of the Murine Coronavirus Genome. J. Virol. 78: 669-682 [Abstract] [Full Text]  
  • Zuniga, S., Sola, I., Alonso, S., Enjuanes, L. (2004). Sequence Motifs Involved in the Regulation of Discontinuous Coronavirus Subgenomic RNA Synthesis. J. Virol. 78: 980-994 [Abstract] [Full Text]  
  • Boot, H. J., Pritz-Verschuren, S. B. E. (2004). Modifications of the 3'-UTR stem-loop of infectious bursal disease virus are allowed without influencing replication or virulence. Nucleic Acids Res 32: 211-222 [Abstract] [Full Text]  
  • Shi, S. T., Yu, G.-Y., Lai, M. M. C. (2003). Multiple Type A/B Heterogeneous Nuclear Ribonucleoproteins (hnRNPs) Can Replace hnRNP A1 in Mouse Hepatitis Virus RNA Synthesis. J. Virol. 77: 10584-10593 [Abstract] [Full Text]  
  • Lo, M. K., Tilgner, M., Bernard, K. A., Shi, P.-Y. (2003). Functional Analysis of Mosquito-Borne Flavivirus Conserved Sequence Elements within 3' Untranslated Region of West Nile Virus by Use of a Reporting Replicon That Differentiates between Viral Translation and RNA Replication. J. Virol. 77: 10004-10014 [Abstract] [Full Text]  
  • Escors, D., Izeta, A., Capiscol, C., Enjuanes, L. (2003). Transmissible Gastroenteritis Coronavirus Packaging Signal Is Located at the 5' End of the Virus Genome. J. Virol. 77: 7890-7902 [Abstract] [Full Text]  
  • Dollenmaier, G., Weitz, M. (2003). Interaction of glyceraldehyde-3-phosphate dehydrogenase with secondary and tertiary RNA structural elements of the hepatitis A virus 3' translated and non-translated regions. J. Gen. Virol. 84: 403-414 [Abstract] [Full Text]  
  • Lopez de Quinto, S., Saiz, M., de la Morena, D., Sobrino, F., Martinez-Salas, E. (2002). IRES-driven translation is stimulated separately by the FMDV 3'-NCR and poly(A) sequences. Nucleic Acids Res 30: 4398-4405 [Abstract] [Full Text]  
  • Friebe, P., Bartenschlager, R. (2002). Genetic Analysis of Sequences in the 3' Nontranslated Region of Hepatitis C Virus That Are Important for RNA Replication. J. Virol. 76: 5326-5338 [Abstract] [Full Text]  
  • Liu, Q., Johnson, R. F., Leibowitz, J. L. (2001). Secondary Structural Elements within the 3' Untranslated Region of Mouse Hepatitis Virus Strain JHM Genomic RNA. J. Virol. 75: 12105-12113 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»