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Journal of Virology, June 2001, p. 4964-4972, Vol. 75, No. 11
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.11.4964-4972.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Construction and Analysis of an Infectious Human Immunodeficiency
Virus Type 1 Subtype C Molecular Clone
Thumbi
Ndung'u,
Boris
Renjifo, and
Max
Essex*
Harvard AIDS Institute and Department of
Immunology and Infectious Diseases, Harvard School of Public
Health, Boston, Massachusetts 02115
Received 27 December 2000/Accepted 5 March 2001
Human immunodeficiency virus type 1 (HIV-1) subtype C is now the
predominant subtype in the global epidemic. This subtype is encountered
in southern Africa and parts of Asia, where the epidemic is rapidly
spreading. One possible explanation for these epidemiological
observations is that this subtype has genetic characteristics that may
contribute to its spread and/or pathogenic potential. In this report,
we describe the construction of MJ4, an infectious chimeric molecular
clone of HIV-1 subtype C that replicates in donor peripheral blood
mononuclear cells and macrophages. We also tested this clone for its
ability to use the chemokine receptors CCR1, CCR2b, CCR3, CXCR4, and
CCR5 and found that the clone utilizes only CCR5 as the coreceptor for
cell entry. The MJ4 clone will be useful in further biological and
virological characterization of HIV-1 subtype C and will be an
important tool in the continuing efforts to understand what may
constitute protective immunity in HIV-1. The clone may also be used in
experimental design of vaccine candidates that may be directed against
HIV-1 subtype C.
*
Corresponding author. Mailing address: Harvard AIDS
Institute and the Department of Immunology and Infectious Diseases,
Harvard School of Public Health, Boston, MA 02115. Phone: (617)
432-0975. Fax: (617) 739-8348. E-mail:
messex{at}hsph.harvard.edu.
Journal of Virology, June 2001, p. 4964-4972, Vol. 75, No. 11
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.11.4964-4972.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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