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Journal of Virology, June 2001, p. 4964-4972, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.4964-4972.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Construction and Analysis of an Infectious Human Immunodeficiency Virus Type 1 Subtype C Molecular Clone

Thumbi Ndung'u, Boris Renjifo, and Max Essex*

Harvard AIDS Institute and Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115

Received 27 December 2000/Accepted 5 March 2001

Human immunodeficiency virus type 1 (HIV-1) subtype C is now the predominant subtype in the global epidemic. This subtype is encountered in southern Africa and parts of Asia, where the epidemic is rapidly spreading. One possible explanation for these epidemiological observations is that this subtype has genetic characteristics that may contribute to its spread and/or pathogenic potential. In this report, we describe the construction of MJ4, an infectious chimeric molecular clone of HIV-1 subtype C that replicates in donor peripheral blood mononuclear cells and macrophages. We also tested this clone for its ability to use the chemokine receptors CCR1, CCR2b, CCR3, CXCR4, and CCR5 and found that the clone utilizes only CCR5 as the coreceptor for cell entry. The MJ4 clone will be useful in further biological and virological characterization of HIV-1 subtype C and will be an important tool in the continuing efforts to understand what may constitute protective immunity in HIV-1. The clone may also be used in experimental design of vaccine candidates that may be directed against HIV-1 subtype C.

* Corresponding author. Mailing address: Harvard AIDS Institute and the Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115. Phone: (617) 432-0975. Fax: (617) 739-8348. E-mail: messex{at}hsph.harvard.edu.

Journal of Virology, June 2001, p. 4964-4972, Vol. 75, No. 11
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.11.4964-4972.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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