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Journal of Virology, May 2001, p. 4936-4940, Vol. 75, No. 10
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.10.4936-4940.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of Human Immunodeficiency Virus Type 1 in Saliva and Blood Plasma by V3-Specific Heteroduplex Tracking Assay and Genotype Analyses

Stephanie A. Freel,1 John M. Williams,1 Julie A. E. Nelson,2 Lauren L. Patton,1,2 Susan A. Fiscus,2,3 Ronald Swanstrom,2,3,4 and Diane C. Shugars1,2,3,5,*

School of Dentistry,1 Center for AIDS Research,2 Departments of Microbiology and Immunology,3 and of Biochemistry and Biophysics,4 and Comprehensive Center for Inflammatory Disorders,5 University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599

Received 6 November 2000/Accepted 21 February 2001

The gp120 V3-encoding region of human immunodeficiency virus type 1 (HIV-1) RNA derived from the saliva and blood plasma of 11 individuals was characterized by heteroduplex tracking assay and sequence analyses. R5-like viral variants were identified in both fluids of all subjects. X4-like variants were detected in the plasma and/or saliva of three subjects, indicating that X4-like variants are not excluded from the saliva compartment. Viral subpopulations were similar in both fluids of most subjects, suggesting that HIV-1 in oral fluids and blood may stem from a common source. These findings raise the possibility of using saliva as a noninvasive fluid for evaluating and monitoring viral evolution in infected persons.

* Corresponding author. Mailing address: Department of Dental Ecology, UNC School of Dentistry, CB#7450, University of North Carolina, Chapel Hill, NC 27599-7450. Phone: (919) 966-5310. Fax: (919) 966-6761. E-mail: diane_shugars{at}dentistry.unc.edu.

Journal of Virology, May 2001, p. 4936-4940, Vol. 75, No. 10
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.10.4936-4940.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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