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Journal of Virology, May 2001, p. 4843-4853, Vol. 75, No. 10
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.10.4843-4853.2001

Transcription Program of Human Herpesvirus 8 (Kaposi's Sarcoma-Associated Herpesvirus)

Mini Paulose-Murphy,1 Nguyen-Khoi Ha,1 Chunsheng Xiang,1 Yidong Chen,2 Laura Gillim,1 Robert Yarchoan,1 Paul Meltzer,2 Michael Bittner,2 Jeffrey Trent,2 and Steven Zeichner1,*

HIV and AIDS Malignancy Branch, National Cancer Institute,1 and Cancer Genetics Branch, National Human Genome Research Institute,2 National Institutes of Health, Bethesda, Maryland 20892

Received 15 November 2000/Accepted 16 February 2001

Human herpesvirus 8 (HHV-8), a gammaherpesvirus implicated in Kaposi's sarcoma, primary effusion lymphoma, and Castleman's disease, encodes several pathogenically important cellular homologs. To define the HHV-8 transcription program, RNA obtained from latently infected body cavity-based lymphoma 1 cells induced to undergo lytic replication was used to query a custom HHV-8 DNA microarray containing nearly every known viral open reading frame. The patterns of viral gene expression offer insights into the replication and pathogenic strategies of HHV-8.

* Corresponding author. Mailing address: NIH, NCI, Bldg. 10, Room 10S255, MSC 1868, Bethesda, MD 20892-1868. Phone: (301) 402-3637. Fax: (301) 480-8250. E-mail: zeichner{at}nih.gov.

Journal of Virology, May 2001, p. 4843-4853, Vol. 75, No. 10
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.10.4843-4853.2001


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