This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nappi, F.
Right arrow Articles by Pavlakis, G. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nappi, F.
Right arrow Articles by Pavlakis, G. N.

 Previous Article  |  Next Article 

Journal of Virology, May 2001, p. 4558-4569, Vol. 75, No. 10
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.10.4558-4569.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of a Novel Posttranscriptional Regulatory Element by Using a rev- and RRE-Mutated Human Immunodeficiency Virus Type 1 DNA Proviral Clone as a Molecular Trap

Filomena Nappi,1,dagger Ralf Schneider,1,Dagger Andrei Zolotukhin,2 Sergey Smulevitch,2 Daniel Michalowski,2 Jenifer Bear,2 Barbara K. Felber,2 and George N. Pavlakis1,*

Human Retrovirus Section1 and Human Retrovirus Pathogenesis Section,2 Basic Research Laboratory, National Cancer Institute-Frederick, Frederick, Maryland 21702-1201

Received 27 November 2000/Accepted 20 February 2001

Human immunodeficiency virus (HIV) and all other lentiviruses utilize the essential viral protein Rev, which binds to RRE RNA, to export their unspliced and partially spliced mRNAs from the nucleus. We used a rev- and RRE-defective HIV type 1 (HIV-1) molecular clone in complementation experiments to establish a method for the rapid isolation of posttranscriptional regulatory elements from the mammalian genome by selecting for rescue of virus replication. Viruses rescued by this method contained a novel element with homology to rodent intracisternal A-particle (IAP) retroelements. A functional element was contained within a 247-nucleotide fragment named RNA transport element (RTE), which was able to promote replication of the Rev- and RRE-defective HIV-1 in both human lymphoid cell lines and primary lymphocytes, demonstrating its potent posttranscriptional function. RTE was functional in many cell types, indicating that the cellular factors that recognize RTE are widely expressed and evolutionarily conserved. RTE also promoted RNA export from Xenopus oocyte nuclei. RTE-mediated RNA transport was CRM1 independent, and RTE did not show high affinity for binding to mRNA export factor TAP/NXF1. Since CRM1 and TAP/NXF1 are critical export receptors associated with the two recognized mRNA export pathways, these results suggest that RTE functions via a distinct export mechanism. Taken together, our results identify a novel posttranscriptional control element that uses a conserved cellular export mechanism.

* Corresponding author. Mailing address: Human Retrovirus Section, BRL, Building 535, Room 210, NCI-Frederick, Frederick, MD 21702. Phone: (301) 846-1474. Fax: (301) 846-6368. E-mail: pavlakis{at}ncifcrf.gov.

dagger Present address: Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy.

Dagger Present address: Institute of Experimental Genetics/AG BIODV, GSF-National Research Center for Environment and Health, 85764 Neuherberg/Munich, Germany.

Journal of Virology, May 2001, p. 4558-4569, Vol. 75, No. 10
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.10.4558-4569.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Uranishi, H., Zolotukhin, A. S., Lindtner, S., Warming, S., Zhang, G.-M., Bear, J., Copeland, N. G., Jenkins, N. A., Pavlakis, G. N., Felber, B. K. (2009). The RNA-binding Motif Protein 15B (RBM15B/OTT3) Acts as Cofactor of the Nuclear Export Receptor NXF1. J. Biol. Chem. 284: 26106-26116 [Abstract] [Full Text]  
  • Lindtner, S., Zolotukhin, A. S., Uranishi, H., Bear, J., Kulkarni, V., Smulevitch, S., Samiotaki, M., Panayotou, G., Felber, B. K., Pavlakis, G. N. (2006). RNA-binding Motif Protein 15 Binds to the RNA Transport Element RTE and Provides a Direct Link to the NXF1 Export Pathway. J. Biol. Chem. 281: 36915-36928 [Abstract] [Full Text]  
  • Smulevitch, S., Michalowski, D., Zolotukhin, A. S., Schneider, R., Bear, J., Roth, P., Pavlakis, G. N., Felber, B. K. (2005). Structural and Functional Analysis of the RNA Transport Element, a Member of an Extensive Family Present in the Mouse Genome. J. Virol. 79: 2356-2365 [Abstract] [Full Text]  
  • Yi, R., Bogerd, H. P., Cullen, B. R. (2002). Recruitment of the Crm1 Nuclear Export Factor Is Sufficient To Induce Cytoplasmic Expression of Incompletely Spliced Human Immunodeficiency Virus mRNAs. J. Virol. 76: 2036-2042 [Abstract] [Full Text]  
  • von Gegerfelt, A. S., Liska, V., Li, P.-L., McClure, H. M., Horie, K., Nappi, F., Montefiori, D. C., Pavlakis, G. N., Marthas, M. L., Ruprecht, R. M., Felber, B. K. (2002). Rev-Independent Simian Immunodeficiency Virus Strains Are Nonpathogenic in Neonatal Macaques. J. Virol. 76: 96-104 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»