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Journal of Virology, May 2001, p. 4506-4518, Vol. 75, No. 10
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.10.4506-4518.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

"Rule of Six": How Does the Sendai Virus RNA Polymerase Keep Count?

Diane Vulliémoz and Laurent Roux*

Department of Genetics and Microbiology, University of Geneva Medical School, CMU, CH-1211 Geneva 4, Switzerland

Received 16 October 2000/Accepted 14 February 2001

The "rule of six" stipulates that the Paramyxovirus RNA polymerase efficiently replicates only viral genomes counting 6n + 0 nucleotides. Because the nucleocapsid proteins (N) interact with 6 nucleotides, an exact nucleotide-N match at the RNA 3'-OH end (3'-OH congruence) may be required for recognition of an active replication promoter. Alternatively, assuming that the six positions for the interaction of N with the nucleotides are not equivalent, the nucleotide position relative to N may be critical (N phase context). The replication abilities of various minireplicons, designed so that the 3'-OH congruence could be discriminated from the N phase context, were studied. The results strongly suggest that the application of the rule of six depends on the recognition of nucleotides positioned in the proper N phase context.

* Corresponding author. Mailing address: Department of Genetics and Microbiology, University of Geneva Medical School, CMU, 1 rue Michel-Servet, CH-1211 Geneva 4, Switzerland. Phone: 41-22-702-56-83. Fax: 41-22-702-57-02. E-mail: Laurent.Roux{at}medecine.unige.ch.

Journal of Virology, May 2001, p. 4506-4518, Vol. 75, No. 10
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.10.4506-4518.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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