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Journal of Virology, May 2001, p. 4506-4518, Vol. 75, No. 10
Department of Genetics and Microbiology,
University of Geneva Medical School, CMU, CH-1211 Geneva 4, Switzerland
Received 16 October 2000/Accepted 14 February 2001
The "rule of six" stipulates that the Paramyxovirus
RNA polymerase efficiently replicates only viral genomes counting
6n + 0 nucleotides. Because the nucleocapsid proteins
(N) interact with 6 nucleotides, an exact nucleotide-N match at the RNA
3'-OH end (3'-OH congruence) may be required for recognition of an
active replication promoter. Alternatively, assuming that the six
positions for the interaction of N with the nucleotides are not
equivalent, the nucleotide position relative to N may be critical (N
phase context). The replication abilities of various minireplicons, designed so that the 3'-OH congruence could be discriminated from the N
phase context, were studied. The results strongly suggest that the
application of the rule of six depends on the recognition of
nucleotides positioned in the proper N phase context.
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.10.4506-4518.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
"Rule of Six": How Does the Sendai Virus
RNA Polymerase Keep Count?
*
Corresponding author. Mailing address: Department of
Genetics and Microbiology, University of Geneva Medical School, CMU, 1 rue Michel-Servet, CH-1211 Geneva 4, Switzerland. Phone:
41-22-702-56-83. Fax: 41-22-702-57-02. E-mail:
Laurent.Roux{at}medecine.unige.ch.
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