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Journal of Virology, January 2001, p. 513-521, Vol. 75, No. 1
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.1.513-521.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cooperative Transformation and Coexpression of Bovine Papillomavirus Type 1 E5 and E7 Proteins

Joanna Bohl,1 Bruce Hull,2 and Scott B. Vande Pol1,*

Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106,1 and Department of Surgery, School of Veterinary Science, Ohio State University, Columbus, Ohio 432102

Received 10 July 2000/Accepted 28 September 2000

Productively infected bovine fibropapillomas were examined for bovine papillomavirus type 1 (BPV-1) E7 localization. BPV-1 E7 was observed in the cytoplasm of basal and lower spinous epithelial cells, coexpressed in the cytoplasm of basal cells with the E5 oncoprotein. E7 was also observed in nucleoli throughout the basal and spinous layers but not in the granular cell layer. Ectopic expression of E7 in cultured epithelial cells gave rise to localization similar to that seen in productive fibropapillomas, with cytoplasmic and nucleolar expression observed. Consistent with the coexpression of E7 and E5 in basal keratinocytes, BPV-1 E7 cooperated with E5 as well as E6 in an anchorage independence transformation assay. While E5 is expressed in both basal and superficial differentiating keratinocytes, BPV-1 E7 is only observed in basal and lower spinous epithelial cells. Therefore, BPV-1 E7 may serve to modulate the cellular response of basal epithelial cells to E5 expression.

* Corresponding author. Mailing address: Institute of Pathology, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH 44106. Phone: (216) 368-1679. Fax: (216) 368-1300. E-mail: sbv{at}pop.cwru.edu.

Journal of Virology, January 2001, p. 513-521, Vol. 75, No. 1
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.1.513-521.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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