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Journal of Virology, January 2001, p. 292-302, Vol. 75, No. 1
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.1.292-302.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The E1 Initiator Recognizes Multiple Overlapping Sites in the Papillomavirus Origin of DNA Replication

Grace Chen1,2 and Arne Stenlund1,*

Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724,1 and Graduate Program in Genetics, State University of New York at Stony Brook, Stony Brook, New York 117942

Received 11 July 2000/Accepted 3 October 2000

A common feature of replicator sequences from a variety of organisms is multiple binding sites for an initiator protein. By binding to the replicator, initiators mark the site and contribute to melting or distortion of the DNA. We have defined the recognition sequence for the papillomavirus E1 initiator and determined the arrangement of binding sites in the viral origin of replication. We show that E1 recognizes a hexanucleotide sequence which is present in overlapping arrays in virtually all papillomavirus replicators. Binding of the initiator to these sites would result in the formation of a closely packed array of E1 molecules that wrap around the double helix.

* Corresponding author. Mailing address: Cold Spring Harbor Laboratory, P.O. Box 100, 1 Bungtown Rd., Cold Spring Harbor, NY 11724. Phone: (516) 367-8407. Fax: (516) 367-8454. E-mail: stenlund{at}cshl.org.

Journal of Virology, January 2001, p. 292-302, Vol. 75, No. 1
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.1.292-302.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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