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Journal of Virology, January 2001, p. 151-160, Vol. 75, No. 1
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.1.151-160.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Gps2, a Protein Partner for Human Papillomavirus E6 Proteins

Yan Yan Degenhardt1 and Saul J. Silverstein2,*

Departments of Pharmacology1 and Microbiology,2 Columbia University, New York, New York 10032

Received 21 July 2000/Accepted 10 October 2000

We have used the yeast two-hybrid system to screen a cDNA library prepared from normal human epidermal keratinocytes and identified protein partners for human papilloma virus (HPV) E6 proteins. A clone that encoded Gps2 interacted with E6 proteins from HPVs of high and low oncogenic risk. The specificity of these reactions was verified and the regions of E6 that were required for interaction were mapped. Steady-state and pulse-chase analyses of cells cotransfected with DNAs expressing E6 from either HPV6 or HPV18 and Gps2 demonstrated that the E6 proteins induced the degradation of Gps2 in vivo but not in vitro. Gps2 exhibited transcriptional activation activity, and high-risk E6 suppressed this activity.

* Corresponding author. Mailing address: Department of Microbiology, Columbia University, 701 W. 168th St., New York, NY 10032. Phone: (212) 305-8149. Fax: (212) 305-5106. E-mail: sjs6{at}columbia.edu.

Journal of Virology, January 2001, p. 151-160, Vol. 75, No. 1
0022-538X/01/$04.00+0   DOI: 10.1128/JVI.75.1.151-160.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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