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Journal of Virology, January 2001, p. 100-106, Vol. 75, No. 1
Institut de Biologie Moléculaire des
Plantes, FRE CNRS 2161, Université Louis Pasteur, 67084 Strasbourg Cedex,1 and Station de
Recherches de Pathologie Comparée, INRA-CNRS, 30380 Saint
Christol-lez-Alès,2 France
Received 29 June 2000/Accepted 4 October 2000
Transmission of cauliflower mosaic virus (CaMV) by aphids requires
two viral nonstructural proteins, the open reading frame (ORF) II and
ORF III products (P2 and P3). An interaction between a C-terminal
domain of P2 and an N-terminal domain of P3 is essential for
transmission. Purified particles of CaMV are efficiently transmitted only if aphids, previously fed a P2-containing solution, are allowed to
acquire a preincubated mixture of P3 and virions in a second feed, thus
suggesting a direct interaction between P3 and coat protein. Herein we
demonstrate that P3 directly interacts with purified viral particles
and unassembled coat protein without the need for any other factor and
that P3 mediates the association of P2 with purified virus particles.
The interaction domain of P3 is located in its C-terminal half,
downstream of the P3-P2 interaction domain but overlapping a region
which binds nucleic acids. Mutagenesis of P3 which interferes with the
interaction between P3 and virions is correlated with the loss of
transmission by aphids. Taken together, our results demonstrate that P3
plays a crucial role in the formation of the CaMV transmissible complex by serving as a bridge between P2 and virus particles.
0022-538X/01/$04.00+0 DOI: 10.1128/JVI.75.1.100-106.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Interaction between the Open Reading Frame III
Product and the Coat Protein Is Required for Transmission of
Cauliflower Mosaic Virus by Aphids

*
Corresponding author. Mailing address: Institut de
Biologie Moléculaire des Plantes, FRE CNRS 2161, Université
Louis Pasteur, 12 rue du Général Zimmer, 67084 Strasbourg
Cedex, France. Phone: (33) 03 88 41 72 61. Fax: (33) 03 88 61 44 42. E-mail: pierre.yot{at}ibmp-ulp.u-strasbg.fr.
Present address: UMR Biologie des Organismes et des Populations
Appliquée à la Protection des Plantes, INRA-ENSA, 35653 Le
Rheu Cedex, France.
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