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Journal of Virology, May 2000, p. 4244-4252, Vol. 74, No. 9
Division of Vector-Borne Infectious Diseases,
Centers for Disease Control and Prevention, Public Health Service,
U.S. Department of Health and Human Services, Fort Collins, Colorado
80522
Received 10 November 1999/Accepted 1 February 2000
Plasmid vectors containing Japanese encephalitis virus (JEV)
premembrane (prM) and envelope (E) genes were constructed that expressed prM and E proteins under the control of a cytomegalovirus immediate-early gene promoter. COS-1 cells transformed with this plasmid vector (JE-4B clone) secreted JEV-specific extracellular particles (EPs) into the culture media. Groups of outbred ICR mice were
given one or two doses of recombinant plasmid DNA or two doses of the
commercial vaccine JEVAX. All mice that received one or two doses of
DNA vaccine maintained JEV-specific antibodies 18 months after initial
immunization. JEVAX induced 100% seroconversion in 3-week-old mice;
however, none of the 3-day-old mice had enzyme-linked immunosorbent
assay titers higher than 1:400. Female mice immunized with this DNA
vaccine developed plaque reduction neutralization antibody titers of
between 1:20 and 1:160 and provided 45 to 100% passive protection to
their progeny following intraperitoneal challenge with 5,000 PFU of
virulent JEV strain SA14. Seven-week-old adult mice that had received a
single dose of JEV DNA vaccine when 3 days of age were completely
protected from a 50,000-PFU JEV intraperitoneal challenge. These
results demonstrate that a recombinant plasmid DNA which produced JEV
EPs in vitro is an effective vaccine.
0022-538X/00/$04.00+0
A Single Intramuscular Injection of Recombinant
Plasmid DNA Induces Protective Immunity and Prevents Japanese
Encephalitis in Mice
*
Corresponding author. Mailing address: P.O. Box 2087, Division of Vector-Borne Infectious Diseases, CDC, Foothill Campus, Fort Collins, CO 80522-2087. Phone: (970) 221-6497. Fax: (970) 221-6476. E-mail: gxc7{at}cdc.gov.
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