This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Drake, D. R.
Right arrow Articles by Lukacher, A. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Drake, D. R., III
Right arrow Articles by Lukacher, A. E.

 Previous Article  |  Next Article 

Journal of Virology, May 2000, p. 4093-4101, Vol. 74, No. 9
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Polyomavirus-Infected Dendritic Cells Induce Antiviral CD8+ T Lymphocytes

Donald R. Drake III,1 Janice M. Moser,1 Annette Hadley,1 John D. Altman,2,3 Charles Maliszewski,4 Eric Butz,4 and Aron E. Lukacher1,*

Department of Pathology,1 Department of Microbiology and Immunology,2 and the Emory Vaccine Center,3 Emory University School of Medicine, Atlanta, Georgia 30322, and Immunex Corporation, Seattle, Washington 981014

Received 21 December 1999/Accepted 29 January 2000

CD8+ T cells are critical for the clearance of acute polyomavirus infection and the prevention of polyomavirus-induced tumors, but the antigen-presenting cell(s) involved in generating polyomavirus-specific CD8+ T cells have not been defined. We investigated whether dendritic cells and macrophages are permissive for polyomavirus infection and examined their potential for inducing antiviral CD8+ T cells. Although dendritic cells and macrophages both supported productive polyomavirus infection, dendritic cells were markedly more efficient at presenting the immunodominant viral epitope to CD8+ T cells. Additionally, infected dendritic cells, but not infected macrophages, primed anti-polyomavirus CD8+ T cells in vivo. Treatment with Flt3 ligand, a hematopoietic growth factor that dramatically expands the number of dendritic cells, markedly enhanced the magnitude of virus-specific CD8+ T-cell responses during acute infection and the pool of memory anti-polyomavirus CD8+ T cells. These findings suggest that virus-infected dendritic cells induce polyomavirus-specific CD8+ T cells in vivo and raise the potential for their use as cellular adjuvants to promote CD8+ T cell surveillance against polyomavirus-induced tumors.

* Corresponding author. Mailing address: Department of Pathology, Emory University School of Medicine, Woodruff Memorial Research Building, 1639 Pierce Dr., Atlanta, GA 30322. Phone: (404) 727-1896. Fax: (404) 727-5764. E-mail: alukach{at}emory.edu.

Journal of Virology, May 2000, p. 4093-4101, Vol. 74, No. 9
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Shen, X. Z., Lukacher, A. E., Billet, S., Williams, I. R., Bernstein, K. E. (2008). Expression of Angiotensin-converting Enzyme Changes Major Histocompatibility Complex Class I Peptide Presentation by Modifying C Termini of Peptide Precursors. J. Biol. Chem. 283: 9957-9965 [Abstract] [Full Text]  
  • Tegerstedt, K., Lindencrona, J. A., Curcio, C., Andreasson, K., Tullus, C., Forni, G., Dalianis, T., Kiessling, R., Ramqvist, T. (2005). A Single Vaccination with Polyomavirus VP1/VP2Her2 Virus-Like Particles Prevents Outgrowth of HER-2/neu-Expressing Tumors. Cancer Res. 65: 5953-5957 [Abstract] [Full Text]  
  • Comoli, P., Basso, S., Azzi, A., Moretta, A., De Santis, R., Del Galdo, F., De Palma, R., Valente, U., Nocera, A., Perfumo, F., Locatelli, F., Maccario, R., Ginevri, F. (2003). Dendritic Cells Pulsed with Polyomavirus BK Antigen Induce Ex Vivo Polyoma BK Virus-Specific Cytotoxic T-Cell Lines in Seropositive Healthy Individuals and Renal Transplant Recipients. J. Am. Soc. Nephrol. 14: 3197-3204 [Abstract] [Full Text]  
  • Sailaja, G., Husain, S., Nayak, B. P., Jabbar, A. M. (2003). Long-Term Maintenance of gp120-Specific Immune Responses by Genetic Vaccination with the HIV-1 Envelope Genes Linked to the Gene Encoding Flt-3 Ligand. J. Immunol. 170: 2496-2507 [Abstract] [Full Text]  
  • Vacheron, S., Luther, S. A., Acha-Orbea, H. (2002). Preferential Infection of Immature Dendritic Cells and B Cells by Mouse Mammary Tumor Virus. J. Immunol. 168: 3470-3476 [Abstract] [Full Text]  
  • Rudolf, M. P., Fausch, S. C., Da Silva, D. M., Kast, W. M. (2001). Human Dendritic Cells Are Activated by Chimeric Human Papillomavirus Type-16 Virus-Like Particles and Induce Epitope-Specific Human T Cell Responses In Vitro. J. Immunol. 166: 5917-5924 [Abstract] [Full Text]  
  • Moser, J. M., Altman, J. D., Lukacher, A. E. (2001). Antiviral Cd8+ T Cell Responses in Neonatal Mice: Susceptibility to Polyoma Virus-Induced Tumors Is Associated with Lack of Cytotoxic Function by Viral Antigen-Specific T Cells. JEM 193: 595-606 [Abstract] [Full Text]  
  • Drake, D. R. III, Shawver, M. L., Hadley, A., Butz, E., Maliszewski, C., Lukacher, A. E. (2001). Induction of Polyomavirus-Specific CD8+ T Lymphocytes by Distinct Dendritic Cell Subpopulations. J. Virol. 75: 544-547 [Abstract] [Full Text]  
  • Ignatius, R., Marovich, M., Mehlhop, E., Villamide, L., Mahnke, K., Cox, W. I., Isdell, F., Frankel, S. S., Mascola, J. R., Steinman, R. M., Pope, M. (2000). Canarypox Virus-Induced Maturation of Dendritic Cells Is Mediated by Apoptotic Cell Death and Tumor Necrosis Factor Alpha Secretion. J. Virol. 74: 11329-11338 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»