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Journal of Virology, May 2000, p. 3967-3974, Vol. 74, No. 9
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Clinical Isolates of Measles Virus Use CD46 as a Cellular Receptordagger

Marianne Manchester,1,* Danelle S. Eto,1 Alexandra Valsamakis,1,Dagger Paloma B. Liton,2 Rafael Fernandez-Muñoz,2 Paul A. Rota,3 William J. Bellini,3 Donald N. Forthal,4 and Michael B. A. Oldstone1

Department of Neuropharmacology, The Scripps Research Institute, La Jolla, California 920371; Molecular Virology Laboratory, Hospital "Ramon y Cajal," Instituto Nacional de la Salud, Madrid 28034, Spain2; Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303333; and Department of Medicine, University of California, Irvine, College of Medicine, Orange, California 928684

Received 2 August 1999/Accepted 29 January 2000

Laboratory strains of measles viruses (MV), such as Edmonston and Halle, use the complement regulatory protein CD46 as a cell surface receptor. The receptor usage of clinical isolates of MV, however, remains unclear. Receptor usage by primary patient isolates of MV was compared to isolates that had been passaged on a variety of tissue culture cell lines. All of the isolates could infect cells in a CD46-dependent manner, but their tropism was restricted according to cell type (e.g., lymphocytes versus fibroblasts). The results indicate that patient isolates that have not been adapted to tissue culture cell lines use CD46 as a receptor. In addition, passaging primary MV patient isolates in B95-8 cells selected variants that had alternate receptor usage compared to the original isolate. Thus, changes in receptor usage by MV are dependent upon the cell type used for isolation. Furthermore, our results confirm the relevance of the CD46 receptor to natural measles infection.

* Corresponding author. Mailing address: IMM6, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (858) 784-8086. Fax: (858) 784-9981. E-mail: marim{at}scripps.edu.

dagger Publication number 11694-NP from The Scripps Research Institute.

Dagger Present address: Department of Pathology, The Johns Hopkins School of Medicine, Baltimore, MD 21287.

Journal of Virology, May 2000, p. 3967-3974, Vol. 74, No. 9
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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