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Journal of Virology, May 2000, p. 3967-3974, Vol. 74, No. 9
Department of Neuropharmacology, The Scripps
Research Institute, La Jolla, California 920371;
Molecular Virology Laboratory, Hospital "Ramon y Cajal,"
Instituto Nacional de la Salud, Madrid 28034, Spain2; Division of Viral and
Rickettsial Diseases, Centers for Disease Control and Prevention,
Atlanta, Georgia 303333; and
Department of Medicine, University of California, Irvine, College
of Medicine, Orange, California 928684
Received 2 August 1999/Accepted 29 January 2000
Laboratory strains of measles viruses (MV), such as Edmonston and
Halle, use the complement regulatory protein CD46 as a cell surface
receptor. The receptor usage of clinical isolates of MV, however,
remains unclear. Receptor usage by primary patient isolates of MV was
compared to isolates that had been passaged on a variety of tissue
culture cell lines. All of the isolates could infect cells in a
CD46-dependent manner, but their tropism was restricted according to
cell type (e.g., lymphocytes versus fibroblasts). The results indicate
that patient isolates that have not been adapted to tissue culture cell
lines use CD46 as a receptor. In addition, passaging primary MV patient
isolates in B95-8 cells selected variants that had alternate receptor
usage compared to the original isolate. Thus, changes in receptor usage
by MV are dependent upon the cell type used for isolation. Furthermore, our results confirm the relevance of the CD46 receptor to natural measles infection.
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Clinical Isolates of Measles Virus Use CD46 as a Cellular
Receptor

*
Corresponding author. Mailing address: IMM6, The
Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla,
CA 92037. Phone: (858) 784-8086. Fax: (858) 784-9981. E-mail:
marim{at}scripps.edu.
Publication number 11694-NP from The Scripps Research Institute.
Present address: Department of Pathology, The Johns Hopkins School
of Medicine, Baltimore, MD 21287.
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