This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kung, S. K. P.
Right arrow Articles by Chen, I. S. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kung, S. K. P.
Right arrow Articles by Chen, I. S. Y.

 Previous Article  |  Next Article 

Journal of Virology, April 2000, p. 3668-3681, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Murine Leukemia Virus (MuLV) Long Terminal Repeat Derived from Rhesus Macaques in the Context of a Lentivirus Vector and MuLV gag Sequence Results in High-Level Gene Expression in Human T Lymphocytes

Sam K. P. Kung, Dong Sung An, and Irvin S. Y. Chen*

Departments of Microbiology & Immunology and Medicine, UCLA School of Medicine, Los Angeles, California 90095-1678

Received 7 September 1999/Accepted 19 January 2000

We constructed human immunodeficiency virus type 1 (HIV-1) vectors that will allow higher levels of gene expression in T cells. Gene expression under the control of an internal cytomegalovirus (CMV) immediate-early promoter in a self-inactivating lentiviral vector (CSCG) is 4- to 15-fold lower in T-cell lines (SUPT1 and CEMX174) than in non-lymphoid-cell lines (HeLa and 293T). This is in contrast to a Moloney murine leukemia virus (MoMLV)-based retrovirus vector (SRalpha LEGFP). We therefore replaced the internal CMV promoter of CSCG with three different murine oncoretroviral long terminal repeat (LTR) promoters---murine sarcoma virus (MSV), MoMLV (MLV), and the LTR (termed Rh-MLV) that is derived from the ampho-mink cell focus-forming (AMP/MCF) retrovirus in the serum of one rhesus macaque monkey that developed T-cell lymphoma following autologous transplantation of enriched bone marrow stem cells transduced with a retrovirus vector preparation containing replication-competent viruses (E. F. Vanin, M. Kaloss, C. Broscius, and A. W. Nienhuis, J. Virol. 68:4241-4250, 1994). We found that the combination of Rh-MLV LTR and a partial gag sequence of MoMLV (Delta gag871-1612) in CS-Rh-MLV-E gave the highest level of enhanced green fluorescent protein (EGFP) gene expression compared with MLV, MSV LTR, phosphoglycerate kinase, and CMV promoters in T-cell lines, as well as activated primary T cells. Interestingly, there was a further two- to threefold increase in EGFP expression (thus, 10-fold-higher expression than with CMV) when the Rh-MLV promoter and Delta gag871-1612 were used in a self-inactivating-vector setting that has a further deletion in the U3 region of the HIV-1 LTR. These hybrid vectors should prove useful in gene therapy applications for T cells.

* Corresponding author. Mailing address: Departments of Microbiology & Immunology and Medicine, UCLA School of Medicine, 10833 Le Conte Ave., 11-934 Factor Building, Los Angeles, CA 90095-1678. Phone: (310) 825-4793. Fax: (310) 794-7682. E-mail: rtaweesu{at}ucla.edu.

Journal of Virology, April 2000, p. 3668-3681, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Saleh, A., Shan, L., Halayko, A. J., Kung, S., Gounni, A. S. (2009). Critical Role for STAT3 in IL-17A-Mediated CCL11 Expression in Human Airway Smooth Muscle Cells. J. Immunol. 182: 3357-3365 [Abstract] [Full Text]  
  • Li, Y., Chen, S., Yuan, J., Yang, Y., Li, J., Ma, J., Wu, X., Freund, M., Pollok, K., Hanenberg, H., Goebel, W. S., Yang, F.-C. (2009). Mesenchymal stem/progenitor cells promote the reconstitution of exogenous hematopoietic stem cells in Fancg-/- mice in vivo. Blood 113: 2342-2351 [Abstract] [Full Text]  
  • An, D. S., Donahue, R. E., Kamata, M., Poon, B., Metzger, M., Mao, S.-H., Bonifacino, A., Krouse, A. E., Darlix, J.-L., Baltimore, D., Qin, F. X.-F., Chen, I. S. Y. (2007). Stable reduction of CCR5 by RNAi through hematopoietic stem cell transplant in non-human primates. Proc. Natl. Acad. Sci. USA 104: 13110-13115 [Abstract] [Full Text]  
  • Farmen, S. L., Karp, P. H., Ng, P., Palmer, D. J., Koehler, D. R., Hu, J., Beaudet, A. L., Zabner, J., Welsh, M. J. (2005). Gene transfer of CFTR to airway epithelia: low levels of expression are sufficient to correct Cl- transport and overexpression can generate basolateral CFTR. Am. J. Physiol. Lung Cell. Mol. Physiol. 289: L1123-L1130 [Abstract] [Full Text]  
  • Chang, M., Brown, H. J., Collado-Hidalgo, A., Arevalo, J. M., Galic, Z., Symensma, T. L., Tanaka, L., Deng, H., Zack, J. A., Sun, R., Cole, S. W. (2005). {beta}-Adrenoreceptors Reactivate Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication via PKA-Dependent Control of Viral RTA. J. Virol. 79: 13538-13547 [Abstract] [Full Text]  
  • Poon, B., Chen, I. S. Y. (2003). Human Immunodeficiency Virus Type 1 (HIV-1) Vpr Enhances Expression from Unintegrated HIV-1 DNA. J. Virol. 77: 3962-3972 [Abstract] [Full Text]  
  • Morizono, K., Bristol, G., Xie, Y.-m., Kung, S. K.-P., Chen, I. S. Y. (2001). Antibody-Directed Targeting of Retroviral Vectors via Cell Surface Antigens. J. Virol. 75: 8016-8020 [Abstract] [Full Text]  
  • Choi, J. K., Hoang, N., Vilardi, A. M., Conrad, P., Emerson, S. G., Gewirtz, A. M. (2001). Hybrid HIV/MSCV LTR Enhances Transgene Expression of Lentiviral Vectors in Human CD34+ Hematopoietic Cells. Stem Cells 19: 236-246 [Abstract] [Full Text]  
  • Gao, Z., Golob, J., Tanavde, V. M., Civin, C. I., Hawley, R. G., Cheng, L. (2001). High Levels of Transgene Expression Following Transduction of Long-Term NOD/SCID-Repopulating Human Cells with a Modified Lentiviral Vector. Stem Cells 19: 247-259 [Abstract] [Full Text]  
  • An, D. S., Kung, S. K. P., Bonifacino, A., Wersto, R. P., Metzger, M. E., Agricola, B. A., Mao, S. H., Chen, I. S. Y., Donahue, R. E. (2001). Lentivirus Vector-Mediated Hematopoietic Stem Cell Gene Transfer of Common Gamma-Chain Cytokine Receptor in Rhesus Macaques. J. Virol. 75: 3547-3555 [Abstract] [Full Text]  
  • An, D. S., Xie, Y.-m., Chen, I. S. Y. (2001). Envelope Gene of the Human Endogenous Retrovirus HERV-W Encodes a Functional Retrovirus Envelope. J. Virol. 75: 3488-3489 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»