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Journal of Virology, April 2000, p. 3478-3485, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of Antigenic Proteins Encoded by Human Herpesvirus 8 and Seroprevalence in the General Population and among Patients with and without Kaposi's Sarcoma

Harutaka Katano,1 Takuya Iwasaki,1 Nobuyoshi Baba,1 Masanori Terai,1 Shigeo Mori,2 Aikichi Iwamoto,3 Takeshi Kurata,1 and Tetsutaro Sata1,4,*

Department of Pathology1 and Laboratory of Pathology,4 AIDS Research Center, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, and Departments of Pathology2 and Infectious Diseases,3 Institute of Medical Science, University of Tokyo, Minato, Tokyo 108-8639, Japan

Received 15 November 1999/Accepted 19 January 2000

To establish a sensitive and specific antibody assay, potent antigenic proteins encoded by human herpesvirus 8 (HHV8) were studied. Fifteen recombinant HHV8-encoded proteins were produced as glutathione S-transferase fusion proteins. The sera from AIDS-associated Kaposi's sarcoma (KS) patients reacted with four proteins encoded by open reading frames (ORFs) K8.1, 59, 65, and 73 in a Western blot assay. An enzyme-linked immunosorbent assay (ELISA) using these four proteins as antigens (mixed-antigen ELISA) revealed that all 26 sera derived from KS patients (24 with and 2 without human immunodeficiency virus infection) became positive for anti-HHV8 antibodies. The presence of HHV8 was demonstrated in 14 (1.4%) of 1,004 sera from the Japanese general population and 10 (1.9%) of 527 sera from patients without HHV8-associated diseases. The presence of immunoglobulin G (IgG) and IgM antibodies against HHV8 examined further by the mixed-antigen ELISA and Western blotting revealed IgG antibody in all ELISA-positive sera, while IgM antibody against ORF K8.1 was absent. These data suggest that the ORF 73 and 65 proteins are potent antigens for a sensitive serological assay.

* Corresponding author. Mailing address: Laboratory of Pathology, AIDS Research Center, and Department of Pathology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan. Phone: 81-3-5285-1111, ext. 2627. Fax: 81-3-5285-1189. E-mail: tsata{at}nih.go.jp.

Journal of Virology, April 2000, p. 3478-3485, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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