Previous Article | Next Article 
Journal of Virology, April 2000, p. 3470-3477, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
NP and L Proteins of Lymphocytic Choriomeningitis Virus (LCMV)
Are Sufficient for Efficient Transcription and Replication of LCMV
Genomic RNA Analogs
Ki Jeong
Lee,
Isabel S.
Novella,
Michael N.
Teng,§
Michael B. A.
Oldstone, and
Juan Carlos
de la
Torre*
Division of Virology, Department of
Neuropharmacology, The Scripps Research Institute, La Jolla,
California 92037
Received 23 September 1999/Accepted 13 January 2000
The genome of lymphocytic choriomeningitis virus (LCMV) consists of
two negative-sense single-stranded RNA segments, designated L and S. Both segments contain two viral genes in an ambisense coding strategy,
with the genes being separated by an intergenic region (IGR). We have
developed a reverse genetic system that allows the investigation of
cis-acting signals and trans-acting factors
involved in transcription and replication of LCMV. To this end, we
constructed an LCMV S minigenome consisting of a negative-sense copy of
the chloramphenicol acetyltransferase (CAT) reporter gene flanked
upstream by the S 5' untranslated region (UTR) and IGR and downstream
by the S 3' UTR. CAT expression was detected in LCMV-infected cells
transfected with the minigenome RNA. Intracellular coexpression of the
LCMV minigenome and LCMV L and NP proteins supplied from cotransfected
plasmids driven by the T7 RNA polymerase provided by the recombinant
vaccinia virus vTF7-3 resulted in high levels of CAT activity and
synthesis of subgenomic CAT mRNA and antiminigenome RNA
species. Thus, L and NP represent the minimal viral
trans-acting factors required for efficient RNA synthesis
mediated by LCMV polymerase.
*
Corresponding author. Mailing address: Imm-6, Division
of Virology, Department of Neuropharmacology, The Scripps
Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA
92037. Phone: (858) 784-6462. Fax: (858) 784-9981. E-mail:
juanct{at}scripps.edu.

Publication 12708-NP from The Scripps Research
Institute.

Present address: Medical College of Ohio, Toledo, OH
43614.
§
Present address: LID/NIAID, Bethesda, MD 20892-0720.
Journal of Virology, April 2000, p. 3470-3477, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Albarino, C. G., Bergeron, E., Erickson, B. R., Khristova, M. L., Rollin, P. E., Nichol, S. T.
(2009). Efficient Reverse Genetics Generation of Infectious Junin Viruses Differing in Glycoprotein Processing. J. Virol.
83: 5606-5614
[Abstract]
[Full Text]
-
Emonet, S. F., Garidou, L., McGavern, D. B., de la Torre, J. C.
(2009). Generation of recombinant lymphocytic choriomeningitis viruses with trisegmented genomes stably expressing two additional genes of interest. Proc. Natl. Acad. Sci. USA
106: 3473-3478
[Abstract]
[Full Text]
-
Hass, M., Lelke, M., Busch, C., Becker-Ziaja, B., Gunther, S.
(2008). Mutational Evidence for a Structural Model of the Lassa Virus RNA Polymerase Domain and Identification of Two Residues, Gly1394 and Asp1395, That Are Critical for Transcription but Not Replication of the Genome. J. Virol.
82: 10207-10217
[Abstract]
[Full Text]
-
Chen, M., Lan, S., Ou, R., Price, G. E., Jiang, H., de la Torre, J. C., Moskophidis, D.
(2008). Genomic and biological characterization of aggressive and docile strains of lymphocytic choriomeningitis virus rescued from a plasmid-based reverse-genetics system. J. Gen. Virol.
89: 1421-1433
[Abstract]
[Full Text]
-
Rojek, J. M., Campbell, K. P., Oldstone, M. B.A., Kunz, S.
(2007). Old World Arenavirus Infection Interferes with the Expression of Functional {alpha}-Dystroglycan in the Host Cell. Mol. Biol. Cell
18: 4493-4507
[Abstract]
[Full Text]
-
Ellenberg, P., Linero, F. N., Scolaro, L. A.
(2007). Superinfection exclusion in BHK-21 cells persistently infected with Junin virus. J. Gen. Virol.
88: 2730-2739
[Abstract]
[Full Text]
-
Capul, A. A., Perez, M., Burke, E., Kunz, S., Buchmeier, M. J., de la Torre, J. C.
(2007). Arenavirus Z-Glycoprotein Association Requires Z Myristoylation but Not Functional RING or Late Domains. J. Virol.
81: 9451-9460
[Abstract]
[Full Text]
-
Hass, M., Westerkofsky, M., Muller, S., Becker-Ziaja, B., Busch, C., Gunther, S.
(2006). Mutational Analysis of the Lassa Virus Promoter. J. Virol.
80: 12414-12419
[Abstract]
[Full Text]
-
Flatz, L., Bergthaler, A., de la Torre, J. C., Pinschewer, D. D.
(2006). Recovery of an arenavirus entirely from RNA polymerase I/II-driven cDNA. Proc. Natl. Acad. Sci. USA
103: 4663-4668
[Abstract]
[Full Text]
-
Sanchez, A. B., Perez, M., Cornu, T., de la Torre, J. C.
(2005). RNA Interference-Mediated Virus Clearance from Cells both Acutely and Chronically Infected with the Prototypic Arenavirus Lymphocytic Choriomeningitis Virus. J. Virol.
79: 11071-11081
[Abstract]
[Full Text]
-
Sanchez, A. B., de la Torre, J. C.
(2005). Genetic and Biochemical Evidence for an Oligomeric Structure of the Functional L Polymerase of the Prototypic Arenavirus Lymphocytic Choriomeningitis Virus. J. Virol.
79: 7262-7268
[Abstract]
[Full Text]
-
Groseth, A., Feldmann, H., Theriault, S., Mehmetoglu, G., Flick, R.
(2005). RNA Polymerase I-Driven Minigenome System for Ebola Viruses. J. Virol.
79: 4425-4433
[Abstract]
[Full Text]
-
Pinschewer, D. D., Perez, M., de la Torre, J. C.
(2005). Dual Role of the Lymphocytic Choriomeningitis Virus Intergenic Region in Transcription Termination and Virus Propagation. J. Virol.
79: 4519-4526
[Abstract]
[Full Text]
-
Schneider, U., Blechschmidt, K., Schwemmle, M., Staeheli, P.
(2004). Overlap of Interaction Domains Indicates a Central Role of the P Protein in Assembly and Regulation of the Borna Disease Virus Polymerase Complex. J. Biol. Chem.
279: 55290-55296
[Abstract]
[Full Text]
-
Hass, M., Golnitz, U., Muller, S., Becker-Ziaja, B., Gunther, S.
(2004). Replicon System for Lassa Virus. J. Virol.
78: 13793-13803
[Abstract]
[Full Text]
-
Perez, M., Greenwald, D. L., de La Torre, J. C.
(2004). Myristoylation of the RING Finger Z Protein Is Essential for Arenavirus Budding. J. Virol.
78: 11443-11448
[Abstract]
[Full Text]
-
Cornu, T. I., Feldmann, H., de la Torre, J. C.
(2004). Cells Expressing the RING Finger Z Protein Are Resistant to Arenavirus Infection. J. Virol.
78: 2979-2983
[Abstract]
[Full Text]
-
Schneider, U., Naegele, M., Staeheli, P., Schwemmle, M.
(2003). Active Borna Disease Virus Polymerase Complex Requires a Distinct Nucleoprotein-to-Phosphoprotein Ratio but No Viral X Protein. J. Virol.
77: 11781-11789
[Abstract]
[Full Text]
-
Perez, M., Craven, R. C., de la Torre, J. C.
(2003). The small RING finger protein Z drives arenavirus budding: Implications for antiviral strategies. Proc. Natl. Acad. Sci. USA
100: 12978-12983
[Abstract]
[Full Text]
-
Jacamo, R., Lopez, N., Wilda, M., Franze-Fernandez, M. T.
(2003). Tacaribe Virus Z Protein Interacts with the L Polymerase Protein To Inhibit Viral RNA Synthesis. J. Virol.
77: 10383-10393
[Abstract]
[Full Text]
-
Pinschewer, D. D., Perez, M., Sanchez, A. B., de la Torre, J. C.
(2003). Recombinant lymphocytic choriomeningitis virus expressing vesicular stomatitis virus glycoprotein. Proc. Natl. Acad. Sci. USA
100: 7895-7900
[Abstract]
[Full Text]
-
Pinschewer, D. D., Perez, M., de la Torre, J. C.
(2003). Role of the Virus Nucleoprotein in the Regulation of Lymphocytic Choriomeningitis Virus Transcription and RNA Replication. J. Virol.
77: 3882-3887
[Abstract]
[Full Text]
-
Perez, M., de la Torre, J. C.
(2002). Characterization of the Genomic Promoter of the Prototypic Arenavirus Lymphocytic Choriomeningitis Virus. J. Virol.
77: 1184-1194
[Abstract]
[Full Text]
-
Neumann, G., Whitt, M. A., Kawaoka, Y.
(2002). A decade after the generation of a negative-sense RNA virus from cloned cDNA - what have we learned?. J. Gen. Virol.
83: 2635-2662
[Abstract]
[Full Text]
-
Cornu, T. I., de la Torre, J. C.
(2002). Characterization of the Arenavirus RING Finger Z Protein Regions Required for Z-Mediated Inhibition of Viral RNA Synthesis. J. Virol.
76: 6678-6688
[Abstract]
[Full Text]
-
Lee, K. J., Perez, M., Pinschewer, D. D., de la Torre, J. C.
(2002). Identification of the Lymphocytic Choriomeningitis Virus (LCMV) Proteins Required To Rescue LCMV RNA Analogs into LCMV-Like Particles. J. Virol.
76: 6393-6397
[Abstract]
[Full Text]
-
Lopez, N., Jacamo, R., Franze-Fernandez, M. T.
(2001). Transcription and RNA Replication of Tacaribe Virus Genome and Antigenome Analogs Require N and L Proteins: Z Protein Is an Inhibitor of These Processes. J. Virol.
75: 12241-12251
[Abstract]
[Full Text]
-
Cornu, T. I., de la Torre, J. C.
(2001). RING Finger Z Protein of Lymphocytic Choriomeningitis Virus (LCMV) Inhibits Transcription and RNA Replication of an LCMV S-Segment Minigenome. J. Virol.
75: 9415-9426
[Abstract]
[Full Text]
-
Flick, R., Pettersson, R. F.
(2001). Reverse Genetics System for Uukuniemi Virus (Bunyaviridae): RNA Polymerase I-Catalyzed Expression of Chimeric Viral RNAs. J. Virol.
75: 1643-1655
[Abstract]
[Full Text]
-
Smelt, S. C., Borrow, P., Kunz, S., Cao, W., Tishon, A., Lewicki, H., Campbell, K. P., Oldstone, M. B. A.
(2001). Differences in Affinity of Binding of Lymphocytic Choriomeningitis Virus Strains to the Cellular Receptor {alpha}-Dystroglycan Correlate with Viral Tropism and Disease Kinetics. J. Virol.
75: 448-457
[Abstract]
[Full Text]
-
Tortorici, M. A., Ghiringhelli, P. D., Lozano, M. E., Albariño, C. G., Romanowski, V.
(2001). Zinc-binding properties of Junin virus nucleocapsid protein. J. Gen. Virol.
82: 121-128
[Abstract]
[Full Text]
-
Hoffmann, E., Webster, R. G.
(2000). Unidirectional RNA polymerase I-polymerase II transcription system for the generation of influenza A virus from eight plasmids. J. Gen. Virol.
81: 2843-2847
[Abstract]
[Full Text]