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Journal of Virology, April 2000, p. 3455-3463, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

CD4+ T-Cell-Mediated Antiviral Protection of the Upper Respiratory Tract in BALB/c Mice following Parenteral Immunization with a Recombinant Respiratory Syncytial Virus G Protein Fragment

Hélène Plotnicky-Gilquin,1,* Alain Robert,1 Laurent Chevalet,1 Jean-Francois Haeuw,1 Alain Beck,1 Jean-Yves Bonnefoy,1 Christian Brandt,2 Claire-Anne Siegrist,2 Thien Ngoc Nguyen,1 and Ultan F. Power1

Centre d'Immunologie Pierre Fabre, 74 164 St. Julien en Genevois, France,1 and World Health Organization Collaborating Center for Neonatal Vaccinology, Departments of Pathology and Pediatrics, University of Geneva, Switzerland2

Received 7 September 1999/Accepted 13 January 2000

We analyzed the protective mechanisms induced against respiratory syncytial virus subgroup A (RSV-A) infection in the lower and upper respiratory tracts (LRT and URT) of BALB/c mice after intraperitoneal immunization with a recombinant fusion protein incorporating residues 130 to 230 of RSV-A G protein (BBG2Na). Mother-to-offspring antibody (Ab) transfer and adoptive transfer of BBG2Na-primed B cells into SCID mice demonstrated that Abs are important for LRT protection but have no effect on URT infection. In contrast, RSV-A clearance in the URT was achieved in a dose-dependent fashion after adoptive transfer of BBG2Na-primed T cells, while it was abolished in BBG2Na-immunized mice upon in vivo depletion of CD4+, but not CD8+, T cells. Furthermore, the conserved RSV-A G protein cysteines and residues 193 and 194, overlapping the recently identified T helper cell epitope on the G protein (P. W. Tebbey et al., J. Exp. Med. 188:1967-1972, 1998), were found to be essential for URT but not LRT protection. Taken together, these results demonstrate for the first time that CD4+ T cells induced upon parenteral immunization with an RSV G protein fragment play a critical role in URT protection of normal mice against RSV infection.

* Corresponding author. Mailing address: Centre d'Immunologie Pierre Fabre, 5, Av. Napoléon III, 74 164 St. Julien en Genevois, France. Phone: 33.4 50 35 35 45. Fax: 33.4 50 35 35 90. E-mail: helene.plotnicky{at}pierre.fabre.com.

Journal of Virology, April 2000, p. 3455-3463, Vol. 74, No. 8
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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