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Journal of Virology, March 2000, p. 2920-2925, Vol. 74, No. 6
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Enhancement of Immunoglobulin G2a and Cytotoxic T-Lymphocyte Responses by a Booster Immunization with Recombinant Hepatitis C Virus E2 Protein in E2 DNA-Primed Mice

Man Ki Song,1 Seung Woo Lee,1 You Suk Suh,1 Ki Jeong Lee,1 and Young Chul Sung1,2,*

Department of Life Science1 and School of Environmental Engineering,2 Pohang University of Science and Technology, Pohang, Republic of Korea

Received 29 July 1999/Accepted 13 December 1999

The induction of strong cytotoxic T-lymphocyte (CTL) and humoral responses appear to be essential for the elimination of persistently infecting viruses, such as hepatitis C virus (HCV). Here, we tested several vaccine regimens and demonstrate that a combined vaccine regimen, consisting of HCV E2 DNA priming and boosting with recombinant E2 protein, induces the strongest immune responses to HCV E2 protein. This combined vaccine regimen augments E2-specific immunoglobulin G2a (IgG2a) and CD8+ CTL responses to a greater extent than immunizations with recombinant E2 protein and E2 DNA alone, respectively. In addition, the data showed that a protein boost following one DNA priming was also effective, but much less so than those following two DNA primings. These data indicate that sufficient DNA priming is essential for the enhancement of DNA encoded antigen-specific immunity by a booster immunization with recombinant E2 protein. Furthermore, the enhanced CD8+ CTL and IgG2a responses induced by our combined vaccine regimens are closely associated with the protection of BALB/c mice from challenge with modified CT26 tumor cells expressing HCV E2 protein. Together, our results provide important implications for vaccine development for many pathogens, including HCV, which require strong antibody and CTL responses.

* Corresponding author. Mailing address: Dept. of Life Science, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang 790-784, Korea. Phone: 82-562-279-2294. Fax: 82-562-279-5544. E-mail: ycsung{at}vision.postech.ac.kr.

Journal of Virology, March 2000, p. 2920-2925, Vol. 74, No. 6
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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