This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An author's correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, W.
Right arrow Articles by Imperiale, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, W.
Right arrow Articles by Imperiale, M. J.

 Previous Article  |  Next Article 

Journal of Virology, March 2000, p. 2687-2693, Vol. 74, No. 6
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Interaction of the Adenovirus IVa2 Protein with Viral Packaging Sequences

Wei Zhang and Michael J. Imperiale*

Department of Microbiology and Immunology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, Michigan 48109-0942

Received 4 October 1999/Accepted 20 December 1999

We have demonstrated previously that the adenovirus L1 52/55-kDa protein binds to the viral IVa2 protein in infected cells. The significance of this interaction was unclear, however, based on the known functions of these two proteins: the 52/55-kDa protein is required for viral DNA packaging, while the IVa2 protein is a transactivator of the major late promoter (MLP). In this report, we have attempted to elucidate a role for each of the two proteins in the other's known function. There is no apparent effect of the 52/55-kDa protein on the interaction of the IVa2 protein with the MLP. Surprisingly, however, we found that the IVa2 protein can interact with the adenoviral packaging signal and that this interaction involves DNA sequences that have previously been demonstrated to be required for packaging.

* Corresponding author. Mailing address: University of Michigan Medical School, 6310 Cancer Center, 1500 E. Medical Center Dr., Ann Arbor, MI 48109-0942. Phone: (734) 763-9162. Fax: (734) 647-9271. E-mail: imperial{at}umich.edu.

Journal of Virology, March 2000, p. 2687-2693, Vol. 74, No. 6
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Morris, S. J., Leppard, K. N. (2009). Adenovirus Serotype 5 L4-22K and L4-33K Proteins Have Distinct Functions in Regulating Late Gene Expression. J. Virol. 83: 3049-3058 [Abstract] [Full Text]  
  • Ostapchuk, P., Hearing, P. (2008). Adenovirus IVa2 Protein Binds ATP. J. Virol. 82: 10290-10294 [Abstract] [Full Text]  
  • Christensen, J. B., Byrd, S. A., Walker, A. K., Strahler, J. R., Andrews, P. C., Imperiale, M. J. (2008). Presence of the Adenovirus IVa2 Protein at a Single Vertex of the Mature Virion. J. Virol. 82: 9086-9093 [Abstract] [Full Text]  
  • Wohl, B. P., Hearing, P. (2008). Role for the L1-52/55K Protein in the Serotype Specificity of Adenovirus DNA Packaging. J. Virol. 82: 5089-5092 [Abstract] [Full Text]  
  • Ewing, S. G., Byrd, S. A., Christensen, J. B., Tyler, R. E., Imperiale, M. J. (2007). Ternary Complex Formation on the Adenovirus Packaging Sequence by the IVa2 and L4 22-Kilodalton Proteins. J. Virol. 81: 12450-12457 [Abstract] [Full Text]  
  • Tyler, R. E., Ewing, S. G., Imperiale, M. J. (2007). Formation of a Multiple Protein Complex on the Adenovirus Packaging Sequence by the IVa2 Protein. J. Virol. 81: 3447-3454 [Abstract] [Full Text]  
  • Ali, H., LeRoy, G., Bridge, G., Flint, S. J. (2007). The Adenovirus L4 33-Kilodalton Protein Binds to Intragenic Sequences of the Major Late Promoter Required for Late Phase-Specific Stimulation of Transcription. J. Virol. 81: 1327-1338 [Abstract] [Full Text]  
  • Ostapchuk, P., Anderson, M. E., Chandrasekhar, S., Hearing, P. (2006). The L4 22-Kilodalton Protein Plays a Role in Packaging of the Adenovirus Genome. J. Virol. 80: 6973-6981 [Abstract] [Full Text]  
  • Perez-Romero, P., Gustin, K. E., Imperiale, M. J. (2006). Dependence of the Encapsidation Function of the Adenovirus L1 52/55-Kilodalton Protein on Its Ability To Bind the Packaging Sequence. J. Virol. 80: 1965-1971 [Abstract] [Full Text]  
  • Ostapchuk, P., Yang, J., Auffarth, E., Hearing, P. (2005). Functional Interaction of the Adenovirus IVa2 Protein with Adenovirus Type 5 Packaging Sequences. J. Virol. 79: 2831-2838 [Abstract] [Full Text]  
  • Perez-Romero, P., Tyler, R. E., Abend, J. R., Dus, M., Imperiale, M. J. (2005). Analysis of the Interaction of the Adenovirus L1 52/55-Kilodalton and IVa2 Proteins with the Packaging Sequence In Vivo and In Vitro. J. Virol. 79: 2366-2374 [Abstract] [Full Text]  
  • Xing, L., Zhang, L., Kessel, J. V., Tikoo, S. K. (2003). Identification of cis-acting sequences required for selective packaging of bovine adenovirus type 3 DNA. J. Gen. Virol. 84: 2947-2956 [Abstract] [Full Text]  
  • Erturk, E., Ostapchuk, P., Wells, S. I., Yang, J., Gregg, K., Nepveu, A., Dudley, J. P., Hearing, P. (2003). Binding of CCAAT Displacement Protein CDP to Adenovirus Packaging Sequences. J. Virol. 77: 6255-6264 [Abstract] [Full Text]  
  • Ostapchuk, P., Hearing, P. (2003). Minimal cis-Acting Elements Required for Adenovirus Genome Packaging. J. Virol. 77: 5127-5135 [Abstract] [Full Text]  
  • Zhang, W., Imperiale, M. J. (2003). Requirement of the Adenovirus IVa2 Protein for Virus Assembly. J. Virol. 77: 3586-3594 [Abstract] [Full Text]  
  • Harada, J. N., Shevchenko, A., Shevchenko, A., Pallas, D. C., Berk, A. J. (2002). Analysis of the Adenovirus E1B-55K-Anchored Proteome Reveals Its Link to Ubiquitination Machinery. J. Virol. 76: 9194-9206 [Abstract] [Full Text]  
  • Zhang, W., Low, J. A., Christensen, J. B., Imperiale, M. J. (2001). Role for the Adenovirus IVa2 Protein in Packaging of Viral DNA. J. Virol. 75: 10446-10454 [Abstract] [Full Text]  
  • Rosa-Calatrava, M., Grave, L., Puvion-Dutilleul, F., Chatton, B., Kedinger, C. (2001). Functional Analysis of Adenovirus Protein IX Identifies Domains Involved in Capsid Stability, Transcriptional Activity, and Nuclear Reorganization. J. Virol. 75: 7131-7141 [Abstract] [Full Text]  
  • Matthews, D. A. (2001). Adenovirus Protein V Induces Redistribution of Nucleolin and B23 from Nucleolus to Cytoplasm. J. Virol. 75: 1031-1038 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»