This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Komatsu, T.
Right arrow Articles by Gotoh, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Komatsu, T.
Right arrow Articles by Gotoh, B.

 Previous Article  |  Next Article 

Journal of Virology, March 2000, p. 2477-2480, Vol. 74, No. 5
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Sendai Virus Blocks Alpha Interferon Signaling to Signal Transducers and Activators of Transcription

Takayuki Komatsu,1,* Kenji Takeuchi,1 Junko Yokoo,2 Yukie Tanaka,3 and Bin Gotoh1

Department of Microbiology,1 Radioisotope Research Institute,2 and Central Research Laboratories,3 Fukui Medical University School of Medicine, Yoshida-gun, Fukui 910-1193, Japan

Received 24 September 1999/Accepted 1 December 1999

We demonstrate here that Sendai virus (SeV) blocks alpha interferon (IFN-alpha ) signaling to signal transducers and activators of transcription (STATs) in HeLa cells. IFN-alpha -stimulated tyrosine phosphorylation of STATs and subsequent formation of the IFN-stimulated gene factor 3 transcription complex were inhibited in SeV-infected cells, resulting in inefficient induction of IFN-stimulated gene products. None of the components of the signaling pathway---type I IFN receptor subunits Jak1, Tyk2, Stat1, Stat2, and p48---was degraded. Moreover, tyrosine phosphorylation of Jak1 in response to IFN-alpha was unaffected at the early phase of infection, suggesting that oligomerization of the receptor subunits proceeded normally. In contrast to Jak1, IFN-alpha -stimulated tyrosine phosphorylation of Tyk2 was partially inhibited. Therefore, this partial inhibition of activation of Tyk2 probably contributes to the subsequent failure in the activation of STATs.

* Corresponding author. Mailing address: Department of Microbiology, Fukui Medical University School of Medicine, Shimoaizuki 23-3, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan. Phone: 81 776 61 8324. Fax: 81 776 61 8104. E-mail: koma{at}fmsrsa.fukui-med.ac.jp.

Journal of Virology, March 2000, p. 2477-2480, Vol. 74, No. 5
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zurney, J., Kobayashi, T., Holm, G. H., Dermody, T. S., Sherry, B. (2009). Reovirus {micro}2 Protein Inhibits Interferon Signaling through a Novel Mechanism Involving Nuclear Accumulation of Interferon Regulatory Factor 9. J. Virol. 83: 2178-2187 [Abstract] [Full Text]  
  • Nishio, M., Ohtsuka, J., Tsurudome, M., Nosaka, T., Kolakofsky, D. (2008). Human Parainfluenza Virus Type 2 V Protein Inhibits Genome Replication by Binding to the L Protein: Possible Role in Promoting Viral Fitness. J. Virol. 82: 6130-6138 [Abstract] [Full Text]  
  • Xu, D., Brumm, K., Zhang, L. (2006). The Latent Membrane Protein 1 of Epstein-Barr Virus (EBV) Primes EBV Latency Cells for Type I Interferon Production. J. Biol. Chem. 281: 9163-9169 [Abstract] [Full Text]  
  • Nishio, M., Tsurudome, M., Ito, M., Ito, Y. (2005). Human Parainfluenza Virus Type 4 Is Incapable of Evading the Interferon-Induced Antiviral Effect. J. Virol. 79: 14756-14768 [Abstract] [Full Text]  
  • Best, S. M., Morris, K. L., Shannon, J. G., Robertson, S. J., Mitzel, D. N., Park, G. S., Boer, E., Wolfinbarger, J. B., Bloom, M. E. (2005). Inhibition of Interferon-Stimulated JAK-STAT Signaling by a Tick-Borne Flavivirus and Identification of NS5 as an Interferon Antagonist. J. Virol. 79: 12828-12839 [Abstract] [Full Text]  
  • Forget, G., Gregory, D. J., Olivier, M. (2005). Proteasome-mediated Degradation of STAT1{alpha} following Infection of Macrophages with Leishmania donovani. J. Biol. Chem. 280: 30542-30549 [Abstract] [Full Text]  
  • Lo, M. S., Brazas, R. M., Holtzman, M. J. (2005). Respiratory Syncytial Virus Nonstructural Proteins NS1 and NS2 Mediate Inhibition of Stat2 Expression and Alpha/Beta Interferon Responsiveness. J. Virol. 79: 9315-9319 [Abstract] [Full Text]  
  • Nishio, M., Tsurudome, M., Ito, M., Garcin, D., Kolakofsky, D., Ito, Y. (2005). Identification of Paramyxovirus V Protein Residues Essential for STAT Protein Degradation and Promotion of Virus Replication. J. Virol. 79: 8591-8601 [Abstract] [Full Text]  
  • Lin, R.-J., Liao, C.-L., Lin, E., Lin, Y.-L. (2004). Blocking of the Alpha Interferon-Induced Jak-Stat Signaling Pathway by Japanese Encephalitis Virus Infection. J. Virol. 78: 9285-9294 [Abstract] [Full Text]  
  • Chee, A. V., Roizman, B. (2004). Herpes Simplex Virus 1 Gene Products Occlude the Interferon Signaling Pathway at Multiple Sites. J. Virol. 78: 4185-4196 [Abstract] [Full Text]  
  • Lin, C.-C., Chou, C.-M., Hsu, Y.-L., Lien, J.-C., Wang, Y.-M., Chen, S.-T., Tsai, S.-C., Hsiao, P.-W., Huang, C.-J. (2004). Characterization of Two Mosquito STATs, AaSTAT and CtSTAT: DIFFERENTIAL REGULATION OF TYROSINE PHOSPHORYLATION AND DNA BINDING ACTIVITY BY LIPOPOLYSACCHARIDE TREATMENT AND BY JAPANESE ENCEPHALITIS VIRUS INFECTION. J. Biol. Chem. 279: 3308-3317 [Abstract] [Full Text]  
  • Yokota, S.-i., Yokosawa, N., Kubota, T., Okabayashi, T., Arata, S., Fujii, N. (2003). Suppression of Thermotolerance in Mumps Virus-infected Cells Is Caused by Lack of HSP27 Induction Contributed by STAT-1. J. Biol. Chem. 278: 41654-41660 [Abstract] [Full Text]  
  • Gotoh, B., Takeuchi, K., Komatsu, T., Yokoo, J. (2003). The STAT2 Activation Process Is a Crucial Target of Sendai Virus C Protein for the Blockade of Alpha Interferon Signaling. J. Virol. 77: 3360-3370 [Abstract] [Full Text]  
  • Park, M.-S., Shaw, M. L., Munoz-Jordan, J., Cros, J. F., Nakaya, T., Bouvier, N., Palese, P., Garcia-Sastre, A., Basler, C. F. (2002). Newcastle Disease Virus (NDV)-Based Assay Demonstrates Interferon-Antagonist Activity for the NDV V Protein and the Nipah Virus V, W, and C Proteins. J. Virol. 77: 1501-1511 [Abstract] [Full Text]  
  • Yokosawa, N., Yokota, S.-i., Kubota, T., Fujii, N. (2002). C-Terminal Region of STAT-1{alpha} Is Not Necessary for Its Ubiquitination and Degradation Caused by Mumps Virus V Protein. J. Virol. 76: 12683-12690 [Abstract] [Full Text]  
  • Andrejeva, J., Poole, E., Young, D. F., Goodbourn, S., Randall, R. E. (2002). The p127 Subunit (DDB1) of the UV-DNA Damage Repair Binding Protein Is Essential for the Targeted Degradation of STAT1 by the V Protein of the Paramyxovirus Simian Virus 5. J. Virol. 76: 11379-11386 [Abstract] [Full Text]  
  • Barnes, B. J., Kellum, M. J., Field, A. E., Pitha, P. M. (2002). Multiple Regulatory Domains of IRF-5 Control Activation, Cellular Localization, and Induction of Chemokines That Mediate Recruitment of T Lymphocytes. Mol. Cell. Biol. 22: 5721-5740 [Abstract] [Full Text]  
  • Kato, A., Ohnishi, Y., Hishiyama, M., Kohase, M., Saito, S., Tashiro, M., Nagai, Y. (2002). The Amino-Terminal Half of Sendai Virus C Protein Is Not Responsible for either Counteracting the Antiviral Action of Interferons or Down-Regulating Viral RNA Synthesis. J. Virol. 76: 7114-7124 [Abstract] [Full Text]  
  • Bossert, B., Conzelmann, K.-K. (2002). Respiratory Syncytial Virus (RSV) Nonstructural (NS) Proteins as Host Range Determinants: a Chimeric Bovine RSV with NS Genes from Human RSV Is Attenuated in Interferon-Competent Bovine Cells. J. Virol. 76: 4287-4293 [Abstract] [Full Text]  
  • Andrejeva, J., Young, D. F., Goodbourn, S., Randall, R. E. (2002). Degradation of STAT1 and STAT2 by the V Proteins of Simian Virus 5 and Human Parainfluenza Virus Type 2, Respectively: Consequences for Virus Replication in the Presence of Alpha/Beta and Gamma Interferons. J. Virol. 76: 2159-2167 [Abstract] [Full Text]  
  • Zhirnov, O. P., Konakova, T. E., Wolff, T., Klenk, H.-D. (2002). NS1 Protein of Influenza A Virus Down-Regulates Apoptosis. J. Virol. 76: 1617-1625 [Abstract] [Full Text]  
  • Samuel, C. E. (2001). Antiviral Actions of Interferons. Clin. Microbiol. Rev. 14: 778-809 [Abstract] [Full Text]  
  • Nishio, M., Tsurudome, M., Ito, M., Kawano, M., Komada, H., Ito, Y. (2001). High Resistance of Human Parainfluenza Type 2 Virus Protein-Expressing Cells to the Antiviral and Anti-Cell Proliferative Activities of Alpha/Beta Interferons: Cysteine-Rich V-Specific Domain Is Required for High Resistance to the Interferons. J. Virol. 75: 9165-9176 [Abstract] [Full Text]  
  • Garcin, D., Curran, J., Itoh, M., Kolakofsky, D. (2001). Longer and Shorter Forms of Sendai Virus C Proteins Play Different Roles in Modulating the Cellular Antiviral Response. J. Virol. 75: 6800-6807 [Abstract] [Full Text]  
  • Kato, A., Ohnishi, Y., Kohase, M., Saito, S., Tashiro, M., Nagai, Y. (2001). Y2, the Smallest of the Sendai Virus C Proteins, Is Fully Capable of both Counteracting the Antiviral Action of Interferons and Inhibiting Viral RNA Synthesis. J. Virol. 75: 3802-3810 [Abstract] [Full Text]  
  • Young, D. F., Chatziandreou, N., He, B., Goodbourn, S., Lamb, R. A., Randall, R. E. (2001). Single Amino Acid Substitution in the V Protein of Simian Virus 5 Differentiates Its Ability To Block Interferon Signaling in Human and Murine Cells. J. Virol. 75: 3363-3370 [Abstract] [Full Text]  
  • Wang, X., Li, M., Zheng, H., Muster, T., Palese, P., Beg, A. A., García-Sastre, A. (2000). Influenza A Virus NS1 Protein Prevents Activation of NF-kappa B and Induction of Alpha/Beta Interferon. J. Virol. 74: 11566-11573 [Abstract] [Full Text]  
  • Garcin, D., Curran, J., Kolakofsky, D. (2000). Sendai Virus C Proteins Must Interact Directly with Cellular Components To Interfere with Interferon Action. J. Virol. 74: 8823-8830 [Abstract] [Full Text]  
  • Goodbourn, S., Didcock, L., Randall, R. E. (2000). Interferons: cell signalling, immune modulation, antiviral response and virus countermeasures. J. Gen. Virol. 81: 2341-2364 [Full Text]  
  • Basler, C. F., Wang, X., Muhlberger, E., Volchkov, V., Paragas, J., Klenk, H.-D., Garcia-Sastre, A., Palese, P. (2000). The Ebola virus VP35 protein functions as a type I IFN antagonist. Proc. Natl. Acad. Sci. USA 97: 12289-12294 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»