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Journal of Virology, March 2000, p. 2477-2480, Vol. 74, No. 5
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Sendai Virus Blocks Alpha Interferon Signaling to
Signal Transducers and Activators of Transcription
Takayuki
Komatsu,1,*
Kenji
Takeuchi,1
Junko
Yokoo,2
Yukie
Tanaka,3 and
Bin
Gotoh1
Department of
Microbiology,1 Radioisotope Research
Institute,2 and Central Research
Laboratories,3 Fukui Medical University
School of Medicine, Yoshida-gun, Fukui 910-1193, Japan
Received 24 September 1999/Accepted 1 December 1999
We demonstrate here that Sendai virus (SeV) blocks alpha interferon
(IFN-
) signaling to signal transducers and activators of
transcription (STATs) in HeLa cells. IFN-
-stimulated tyrosine phosphorylation of STATs and subsequent formation of the IFN-stimulated gene factor 3 transcription complex were inhibited in SeV-infected cells, resulting in inefficient induction of IFN-stimulated gene products. None of the components of the signaling pathway
type I IFN
receptor subunits Jak1, Tyk2, Stat1, Stat2, and p48
was degraded.
Moreover, tyrosine phosphorylation of Jak1 in response to IFN-
was unaffected at the early phase of infection, suggesting that
oligomerization of the receptor subunits proceeded normally. In
contrast to Jak1, IFN-
-stimulated tyrosine phosphorylation of
Tyk2 was partially inhibited. Therefore, this partial inhibition of
activation of Tyk2 probably contributes to the subsequent failure in
the activation of STATs.
*
Corresponding author. Mailing address: Department of
Microbiology, Fukui Medical University School of Medicine, Shimoaizuki 23-3, Matsuoka-cho, Yoshida-gun, Fukui 910-1193, Japan. Phone: 81 776 61 8324. Fax: 81 776 61 8104. E-mail:
koma{at}fmsrsa.fukui-med.ac.jp.
Journal of Virology, March 2000, p. 2477-2480, Vol. 74, No. 5
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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