This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leclercq, I.
Right arrow Articles by Wattel, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leclercq, I.
Right arrow Articles by Wattel, E.

 Previous Article  |  Next Article 

Journal of Virology, March 2000, p. 2305-2312, Vol. 74, No. 5
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Host Sequences Flanking the Human T-Cell Leukemia Virus Type 1 Provirus In Vivo

India Leclercq,1 Franck Mortreux,1 Marielle Cavrois,1,* Arnaud Leroy,2 Antoine Gessain,3 Simon Wain-Hobson,4 and Eric Wattel5,6,*

Unité 524 INSERM, Institut de Recherche sur le Cancer de Lille,1 and Unité d'Oncogenèse Virale, Centre Oscar Lambret,2 Lille, Unité d'Epidémiologie des Virus Oncogènes,3 and Unité de Rétrovirologie Moléculaire,4 Institut Pasteur, Paris, and Unité d'Oncogenèse Virale, UMR5537 CNRS-Université Claude Bernard, Centre Léon Bérard, Lyon,5 and Service des Maladies du Sang, CHU 59037 Lille,6 France

Received 26 March 1999/Accepted 23 November 1999

Human pathogenic retroviruses do not have common loci of integration. However, many factors, such as chromatin structure, transcriptional activity, DNA-protein interaction, CpG methylation, and nucleotide composition of the target sequence, may influence integration site selection. These features have been investigated by in vitro integration reactions or by infection of cell lines with recombinant retroviruses. Less is known about target choice for integration in vivo. The present study was conducted in order to assess the characteristics of cellular sequences targeted for human T-cell leukemia virus type 1 (HTLV-1) integration in vivo. Sequencing integration sites from >= 200 proviruses (19 kb of sequence) isolated from 29 infected individuals revealed that HTLV-1 integration is not random at the level of the nucleotide sequence. The virus was found to integrate in A/T-rich regions with a weak consensus sequence at positions within and without of the hexameric repeat generated during integration. These features were not associated with a preference for integration near active regions or repeat elements of the host chromosomes. Most or all of the regions of the genome appear to be accessible to HTLV-1 integration. As with integration in vitro, integration specificity in vivo seems to be determined by local features rather than by the accessibility of specific regions.

* Corresponding author. Mailing address: Unité d'Oncogenèse Virale, UMR5537-CNRS-Université Claude Bernard, Centre Léon-Bérard, 28, rue Laënnec, 69373 Lyon Cedex 08, France. Phone: 334-78-78-26-69. Fax: 334-78-78-27-17. E-mail: ewattel{at}easynet.fr.

Journal of Virology, March 2000, p. 2305-2312, Vol. 74, No. 5
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Derse, D., Crise, B., Li, Y., Princler, G., Lum, N., Stewart, C., McGrath, C. F., Hughes, S. H., Munroe, D. J., Wu, X. (2007). Human T-Cell Leukemia Virus Type 1 Integration Target Sites in the Human Genome: Comparison with Those of Other Retroviruses. J. Virol. 81: 6731-6741 [Abstract] [Full Text]  
  • Moalic, Y., Blanchard, Y., Felix, H., Jestin, A. (2006). Porcine Endogenous Retrovirus Integration Sites in the Human Genome: Features in Common with Those of Murine Leukemia Virus. J. Virol. 80: 10980-10988 [Abstract] [Full Text]  
  • Konesky, K. L., Nyborg, J. K., Laybourn, P. J. (2006). Tax Abolishes Histone H1 Repression of p300 Acetyltransferase Activity at the Human T-Cell Leukemia Virus Type 1 Promoter. J. Virol. 80: 10542-10553 [Abstract] [Full Text]  
  • Nielsen, A. A., Sorensen, A. B., Schmidt, J., Pedersen, F. S. (2005). Analysis of Wild-Type and Mutant SL3-3 Murine Leukemia Virus Insertions in the c-myc Promoter during Lymphomagenesis Reveals Target Site Hot Spots, Virus-Dependent Patterns, and Frequent Error-Prone Gap Repair. J. Virol. 79: 67-78 [Abstract] [Full Text]  
  • Lemasson, I., Polakowski, N. J., Laybourn, P. J., Nyborg, J. K. (2004). Transcription Regulatory Complexes Bind the Human T-Cell Leukemia Virus 5' and 3' Long Terminal Repeats To Control Gene Expression. Mol. Cell. Biol. 24: 6117-6126 [Abstract] [Full Text]  
  • Laufs, S., Gentner, B., Nagy, K. Z., Jauch, A., Benner, A., Naundorf, S., Kuehlcke, K., Schiedlmeier, B., Ho, A. D., Zeller, W. J., Fruehauf, S. (2003). Retroviral vector integration occurs in preferred genomic targets of human bone marrow-repopulating cells. Blood 101: 2191-2198 [Abstract] [Full Text]  
  • Lemasson, I., Polakowski, N. J., Laybourn, P. J., Nyborg, J. K. (2002). Transcription Factor Binding and Histone Modifications on the Integrated Proviral Promoter in Human T-cell Leukemia Virus-I-infected T-cells. J. Biol. Chem. 277: 49459-49465 [Abstract] [Full Text]  
  • Koiwa, T., Hamano-Usami, A., Ishida, T., Okayama, A., Yamaguchi, K., Kamihira, S., Watanabe, T. (2002). 5'-Long Terminal Repeat-Selective CpG Methylation of Latent Human T-Cell Leukemia Virus Type 1 Provirus In Vitro and In Vivo. J. Virol. 76: 9389-9397 [Abstract] [Full Text]  
  • Jin, Y. F., Ishibashi, T., Nomoto, A., Masuda, M. (2002). Isolation and Analysis of Retroviral Integration Targets by Solo Long Terminal Repeat Inverse PCR. J. Virol. 76: 5540-5547 [Abstract] [Full Text]  
  • Mortreux, F., Leclercq, I., Gabet, A.-S., Leroy, A., Westhof, E., Gessain, A., Wain-Hobson, S., Wattel, E. (2001). Somatic Mutation in Human T-Cell Leukemia Virus Type 1 Provirus and Flanking Cellular Sequences During Clonal Expansion In Vivo. JNCI J Natl Cancer Inst 93: 367-377 [Abstract] [Full Text]  
  • Mortreux, F., Kazanji, M., Gabet, A.-S., de Thoisy, B., Wattel, E. (2001). Two-Step Nature of Human T-Cell Leukemia Virus Type 1 Replication in Experimentally Infected Squirrel Monkeys (Saimiri sciureus). J. Virol. 75: 1083-1089 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»