This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Herzog, E.
Right arrow Articles by Hohn, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Herzog, E.
Right arrow Articles by Hohn, T.

 Previous Article  |  Next Article 

Journal of Virology, March 2000, p. 2073-2083, Vol. 74, No. 5
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Rice Tungro Bacilliform Virus Gene II Product Interacts with the Coat Protein Domain of the Viral Gene III Polyprotein

Etienne Herzog, Orlene Guerra-Peraza,dagger and Thomas Hohn*

Friedrich Miescher Institute, CH-4002 Basel, Switzerland

Received 7 September 1999/Accepted 2 December 1999

Rice tungro bacilliform virus (RTBV) is a plant pararetrovirus whose DNA genome contains four genes encoding three proteins and a large polyprotein. The function of most of the viral proteins is still unknown. To investigate the role of the gene II product (P2), we searched for interactions between this protein and other RTBV proteins. P2 was shown to interact with the coat protein (CP) domain of the viral gene III polyprotein (P3) both in the yeast two-hybrid system and in vitro. Domains involved in the P2-CP association have been identified and mapped on both proteins. To determine the importance of this interaction for viral multiplication, the infectivity of RTBV gene II mutants was investigated by agroinoculation of rice plants. The results showed that virus viability correlates with the ability of P2 to interact with the CP domain of P3. This study suggests that P2 could participate in RTBV capsid assembly.

* Corresponding author. Mailing address: Friedrich Miescher Institute, Maulbeerstrasse 66, CH-4058 Basel, Switzerland. Phone: (061) 6976684. Fax: (061) 6973976. E-mail: hohn{at}fmi.ch.

dagger Permanent address: Centro de Bioplanta, Ciego de Avila, Cuba.

Journal of Virology, March 2000, p. 2073-2083, Vol. 74, No. 5
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Guerra-Peraza, O., Kirk, D., Seltzer, V., Veluthambi, K., Schmit, A. C., Hohn, T., Herzog, E. (2005). Coat proteins of Rice tungro bacilliform virus and Mungbean yellow mosaic virus contain multiple nuclear-localization signals and interact with importin {alpha}. J. Gen. Virol. 86: 1815-1826 [Abstract] [Full Text]  
  • Stavolone, L., Villani, M. E., Leclerc, D., Hohn, T. (2005). A coiled-coil interaction mediates cauliflower mosaic virus cell-to-cell movement. Proc. Natl. Acad. Sci. USA 102: 6219-6224 [Abstract] [Full Text]  
  • Vetter, G., Hily, J.-M., Klein, E., Schmidlin, L., Haas, M., Merkle, T., Gilmer, D. (2004). Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. J. Gen. Virol. 85: 2459-2469 [Abstract] [Full Text]  
  • Stavolone, L., Herzog, E., Leclerc, D., Hohn, T. (2001). Tetramerization Is a Conserved Feature of the Virion-Associated Protein in Plant Pararetroviruses. J. Virol. 75: 7739-7743 [Abstract] [Full Text]  
  • Leh, V., Jacquot, E., Geldreich, A., Haas, M., Blanc, S., Keller, M., Yot, P. (2001). Interaction between the Open Reading Frame III Product and the Coat Protein Is Required for Transmission of Cauliflower Mosaic Virus by Aphids. J. Virol. 75: 100-106 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»