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Journal of Virology, March 2000, p. 2067-2072, Vol. 74, No. 5
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Interaction of the Cauliflower Mosaic Virus Coat Protein with the Pregenomic RNA Leader

Orlene Guerra-Peraza,dagger Marc de Tapia,Dagger Thomas Hohn,* and Maja Hemmings-Mieszczak

Friedrich Miescher-Institut, CH-4002 Basel, Switzerland

Received 18 June 1999/Accepted 2 December 1999

Using the yeast three-hybrid system, the interaction of the Cauliflower mosaic virus (CaMV) pregenomic 35S RNA (pgRNA) leader with the viral coat protein, its precursor, and a series of derivatives was studied. The purine-rich domain in the center of the pgRNA leader was found to specifically interact with the coat protein. The zinc finger motif of the coat protein and the preceding basic domain were essential for this interaction. Removal of the N-terminal portion of the basic domain led to loss of specificity but did not affect the strength of the interaction. Mutations of the zinc finger motif abolished not only the interaction with the RNA but also viral infectivity. In the presence of the very acidic C-terminal domain, which is part of the preprotein but is not present in the mature CP, the interaction with the RNA was undetectable.

* Corresponding author. Mailing address: Friedrich Miescher-Institut, Maulbeerstrasse 66, 4058 Basel, Switzerland. Phone: (061) 6976672. Fax: (061) 6973976. E-mail: hohn{at}fmi.ch.

dagger Permanent address: Centro de Bioplanta, Ciego de Avila, Cuba.

Dagger Present address: IPCB-UMR 7519, 67084 Strasbourg, France.

Journal of Virology, March 2000, p. 2067-2072, Vol. 74, No. 5
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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