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Journal of Virology, February 2000, p. 1781-1786, Vol. 74, No. 4
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Role of the Influenza Virus M1 Protein in Nuclear
Export of Viral Ribonucleoproteins
Matthew
Bui,1,
Elizabeth G.
Wills,2
Ari
Helenius,1,
and
Gary
R.
Whittaker2,*
Department of Cell Biology, Yale University
School of Medicine, New Haven, Connecticut
06510,1 and Department of Microbiology
and Immunology, Cornell University, Ithaca, New York
148532
Received 28 July 1999/Accepted 18 November 1999
The protein kinase inhibitor H7 blocks influenza virus replication,
inhibits production of the matrix protein (M1), and leads to a
retention of the viral ribonucleoproteins (vRNPs) in the nucleus at
late times of infection (K. Martin and A. Helenius, Cell 67:117-130,
1991). We show here that production of assembled vRNPs occurs normally
in H7-treated cells, and we have used H7 as a biochemical tool to trap
vRNPs in the nucleus. When H7 was removed from the cells, vRNP export
was specifically induced in a CHO cell line stably expressing
recombinant M1. Similarly, fusion of cells expressing recombinant M1
from a Semliki Forest virus vector allowed nuclear export of vRNPs.
However, export was not rescued when H7 was present in the cells,
implying an additional role for phosphorylation in this process. The
viral NS2 protein was undetectable in these systems. We conclude that
influenza virus M1 is required to induce vRNP nuclear export but that
cellular phosphorylation is an additional factor.
*
Corresponding author. Mailing address: C5141 Veterinary
Medical Center, Cornell University, Ithaca, NY 14853. Phone: (607) 253-4019. Fax: (607) 253-3384. E-mail: grw7{at}cornell.edu.

Present address: Department of Urology, UCLA Medical Center, Los
Angeles,
Calif.

Present address: Laboratorium für Biochemie, ETH-Zentrum,
CH-8092 Zürich,
Switzerland.
Journal of Virology, February 2000, p. 1781-1786, Vol. 74, No. 4
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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