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Journal of Virology, February 2000, p. 1549-1553, Vol. 74, No. 3
Department of
Pathology1 and AIDS Research
Center,2 National Institute of Infectious
Diseases, Tokyo, and Primate Research Institute, Kyoto
University, Aichi,3 Japan
Received 19 July 1999/Accepted 4 November 1999
Newly discovered TT virus (TTV) is widely distributed in human
populations. To understand more about the relationship between TTV and
its hosts, we tested 400 sera from various nonhuman primates for the
presence of TTV DNA by PCR assay. We collected serum samples from 24 different species of nonhuman primates. TTV DNA was determined by PCR
with primers designed from the 5'-end region of the TTV genome.
Nucleotide sequencing and phylogenetic analysis of viral genomes were
also performed. TTV DNA was detected in 87 of 98 (89%) chimpanzees and
3 of 21 (14%) crab-eating macaques. Nucleotide sequences of the PCR
products obtained from both animals were 80 to 100% identical between
two species. In contrast, the sequences differed from TTV isolates in
humans by 24 to 33% at the nucleotide level and 36 to 50% at the
amino acid level. Phylogenetic analysis demonstrated that all TTV
isolates obtained from simians were distinct from the human TTV
isolates. Furthermore, TTV in simians, but not in humans, was
classified into three different genotypes. Our results indicate that
TTV in simians represents a group different from, but closely related
to, TTV in humans. From these results, we tentatively named this TTV
simian TTV (s-TTV). The existence of the s-TTV will be important in
determining the origin, nature, and transmission of human TTV and may
provide useful animal models for studies of the infection and
pathogenesis of this new DNA virus.
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
TT Virus Infection in Nonhuman Primates and
Characterization of the Viral Genome: Identification of Simian TT
Virus Isolates
*
Corresponding author. Mailing address: Department of
Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan. Phone: (81) 3-5285-1111, ext. 2624. Fax: (81) 3-5285-1189. E-mail: kenjiabe{at}nih.go.jp.
Journal of Virology, February 2000, p. 1549-1553, Vol. 74, No. 3
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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