This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brault, V.
Right arrow Articles by Ziegler-Graff, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brault, V.
Right arrow Articles by Ziegler-Graff, V.

 Previous Article  |  Next Article 

Journal of Virology, February 2000, p. 1140-1148, Vol. 74, No. 3
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Effects of Point Mutations in the Readthrough Domain of the Beet Western Yellows Virus Minor Capsid Protein on Virus Accumulation In Planta and on Transmission by Aphids

V. Brault,1 J. Mutterer,2 D. Scheidecker,2 M. T. Simonis,1 E. Herrbach,1 K. Richards,2 and V. Ziegler-Graff2,*

Station de Recherche "Grandes Cultures," INRA, Colmar 68021 Cedex,1 and Institut de Biologie Moléculaire des Plantes du CNRS et de l'Université Louis Pasteur, Strasbourg,2 France

Received 28 June 1999/Accepted 21 October 1999

Point mutations were introduced into or near five conserved sequence motifs of the readthrough domain of the beet western yellows virus minor capsid protein P74. The mutant virus was tested for its ability to accumulate efficiently in agroinfected plants and to be transmitted by its aphid vector, Myzus persicae. The stability of the mutants in the agroinfected and aphid-infected plants was followed by sequence analysis of the progeny virus. Only the mutation Y201D was found to strongly inhibit virus accumulation in planta following agroinfection, but high accumulation levels were restored by reversion or pseudoreversion at this site. Four of the five mutants were poorly aphid transmissible, but in three cases successful transmission was restored by pseudoreversion or second-site mutations. The same second-site mutations in the nonconserved motif PVT(32-34) were shown to compensate for two distinct primary mutations (R24A and E59A/D60A), one on each side of the PVT sequence. In the latter case, a second-site mutation in the PVT motif restored the ability of the virus to move from the hemocoel through the accessory salivary gland following microinjection of mutant virus into the aphid hemocoel but did not permit virus movement across the epithelium separating the intestine from the hemocoel. Successful movement of the mutant virus across both barriers was accompanied by conversion of A59 to E or T, indicating that distinct features of the readthrough domain in this region operate at different stages of the transmission process.

* Corresponding author. Mailing address: Institut de Biologie Moléculaire des Plantes du CNRS et de l'Université Louis Pasteur, Strasbourg, France. Phone: (33) 388 417257. Fax: (33) 388 614442. E-mail: veronique.ziegler-graff{at}ibmp-ulp.u-strasbg.fr.

Journal of Virology, February 2000, p. 1140-1148, Vol. 74, No. 3
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Peter, K. A., Gildow, F., Palukaitis, P., Gray, S. M. (2009). The C Terminus of the Polerovirus P5 Readthrough Domain Limits Virus Infection to the Phloem. J. Virol. 83: 5419-5429 [Abstract] [Full Text]  
  • Peter, K. A., Liang, D., Palukaitis, P., Gray, S. M. (2008). Small deletions in the potato leafroll virus readthrough protein affect particle morphology, aphid transmission, virus movement and accumulation. J. Gen. Virol. 89: 2037-2045 [Abstract] [Full Text]  
  • Kaplan, I. B., Lee, L., Ripoll, D. R., Palukaitis, P., Gildow, F., Gray, S. M. (2007). Point mutations in the potato leafroll virus major capsid protein alter virion stability and aphid transmission. J. Gen. Virol. 88: 1821-1830 [Abstract] [Full Text]  
  • Brault, V., Perigon, S., Reinbold, C., Erdinger, M., Scheidecker, D., Herrbach, E., Richards, K., Ziegler-Graff, V. (2005). The Polerovirus Minor Capsid Protein Determines Vector Specificity and Intestinal Tropism in the Aphid. J. Virol. 79: 9685-9693 [Abstract] [Full Text]  
  • Ng, J. C. K., Tian, T., Falk, B. W. (2004). Quantitative parameters determining whitefly (Bemisia tabaci) transmission of Lettuce infectious yellows virus and an engineered defective RNA. J. Gen. Virol. 85: 2697-2707 [Abstract] [Full Text]  
  • Wang, H.-H., Yu, H.-H., Wong, S.-M. (2004). Mutation of Phe50 to Ser50 in the 126/183-kDa proteins of Odontoglossum ringspot virus abolishes virus replication but can be complemented and restored by exact reversion. J. Gen. Virol. 85: 2447-2457 [Abstract] [Full Text]  
  • Brault, V., Bergdoll, M., Mutterer, J., Prasad, V., Pfeffer, S., Erdinger, M., Richards, K. E., Ziegler-Graff, V. (2003). Effects of Point Mutations in the Major Capsid Protein of Beet Western Yellows Virus on Capsid Formation, Virus Accumulation, and Aphid Transmission. J. Virol. 77: 3247-3256 [Abstract] [Full Text]  
  • Pfeffer, S., Dunoyer, P., Heim, F., Richards, K. E., Jonard, G., Ziegler-Graff, V. (2002). P0 of Beet Western Yellows Virus Is a Suppressor of Posttranscriptional Gene Silencing. J. Virol. 76: 6815-6824 [Abstract] [Full Text]  
  • Reinbold, C., Gildow, F. E., Herrbach, E., Ziegler-Graff, V., Goncalves, M. C., van den Heuvel, J. F. J. M., Brault, V. (2001). Studies on the role of the minor capsid protein in transport of Beet western yellows virus through Myzus persicae. J. Gen. Virol. 82: 1995-2007 [Abstract] [Full Text]  
  • Rouzé-Jouan, J., Terradot, L., Pasquer, F., Tanguy, S., Giblot Ducray-Bourdin, D. (2001). The passage of Potato leafroll virus through Myzus persicae gut membrane regulates transmission efficiency. J. Gen. Virol. 82: 17-23 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»