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Journal of Virology, February 2000, p. 1114-1123, Vol. 74, No. 3
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Horizontal Transmissible Protection against Myxomatosis and Rabbit Hemorrhagic Disease by Using a Recombinant Myxoma Virus

Juan Bárcena,1 Mónica Morales,1 Belén Vázquez,1 José A. Boga,2 Francisco Parra,2 Javier Lucientes,3 Albert Pagès-Manté,4 José M. Sánchez-Vizcaíno,1 Rafael Blasco,1 and Juan M. Torres1,*

Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid,1 Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias (CSIC), Universidad de Oviedo, 33006 Oviedo,2 Departamento de Patología Animal, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza,3 and Laboratorios Hipra S.A. Amer., 1710 Girona,4 Spain

Received 1 July 1999/Accepted 1 November 1999

We have developed a new strategy for immunization of wild rabbit populations against myxomatosis and rabbit hemorrhagic disease (RHD) that uses recombinant viruses based on a naturally attenuated field strain of myxoma virus (MV). The recombinant viruses expressed the RHDV major capsid protein (VP60) including a linear epitope tag from the transmissible gastroenteritis virus (TGEV) nucleoprotein. Following inoculation, the recombinant viruses induced specific antibody responses against MV, RHDV, and the TGEV tag. Immunization of wild rabbits by the subcutaneous and oral routes conferred protection against virulent RHDV and MV challenges. The recombinant viruses showed a limited horizontal transmission capacity, either by direct contact or in a flea-mediated process, promoting immunization of contact uninoculated animals.

* Corresponding author. Mailing address: Centro de Investigación en Sanidad Animal (CISA-INIA), Valdeolmos, 28130 Madrid, Spain. Phone: 34-91-620 23 00. Fax: 34-91-620 22 47. E-mail: jmtorres{at}inia.es.

Journal of Virology, February 2000, p. 1114-1123, Vol. 74, No. 3
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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