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Journal of Virology, December 2000, p. 11983-11987, Vol. 74, No. 24
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Translation and Replication of Human Rhinovirus Type 14 and Mengovirus in Xenopus Oocytes

Andrea V. Gamarnik, Nina Böddeker, and Raul Andino*

Department of Microbiology and Immunology, University of California, San Francisco, California

Received 7 July 2000/Accepted 14 September 2000

We have previously shown that Xenopus oocytes require coinjection of both poliovirus RNA and HeLa cell extracts to support a complete cycle of viral replication yielding high levels of infectious viral particles. This novel system provides a tool for identifying host factors and for biochemically dissect individual steps that lead to virus production. Here we demonstrate that Xenopus oocytes are able to support replication of other picornaviruses such as human rhinovirus 14 and mengovirus. Unlike poliovirus, microinjection of mengovirus RNA yields high viral titers (about 107 PFU/oocyte) without the need for coinjection of additional cell extracts. In contrast, formation of infectious rhinovirus particles requires coinjection of human cell extracts. We found that one of these human factors is required for efficient rhinovirus translation. Our findings uncover differences in the host factor requirements among members of the picornavirus family and provide the means to identify the human protein(s) involved in rhinovirus production.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Box 0414, University of California, San Francisco, CA 94143-0414. Phone: (415) 502-6358. Fax: (415) 476-0939. E-mail: andino{at}itsa.ucsf.edu.

Journal of Virology, December 2000, p. 11983-11987, Vol. 74, No. 24
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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