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Journal of Virology, December 2000, p. 11626-11635, Vol. 74, No. 24
Aviron, Mountain View, California 94043
Received 7 June 2000/Accepted 11 September 2000
Bovine parainfluenza virus type 3 (bPIV3) is being evaluated as an
intranasal vaccine for protection against human PIV3 (hPIV3). In young
infants, the bPIV3 vaccine appears to be infectious, attenuated,
immunogenic, and genetically stable, which are desirable characteristics for an RNA virus vector. To test the potential of the
bPIV3 vaccine strain as a vector, an infectious DNA clone of bPIV3 was
assembled and recombinant bPIV3 (r-bPIV3) was rescued. r-bPIV3
displayed a temperature-sensitive phenotype for growth in tissue
culture at 39°C and was attenuated in the lungs of Syrian golden
hamsters. In order to test whether r-bPIV3 could serve as a vector, the
fusion and hemagglutinin-neuraminidase genes of bPIV3 were replaced
with those of hPIV3. The resulting bovine/human PIV3 was temperature
sensitive for growth in Vero cells at 37°C. The replication of
bovine/human PIV3 was also restricted in the lungs of hamsters, albeit
not as severely as was observed for r-bPIV3. Despite the attenuation
phenotypes observed for r-bPIV3 and bovine/human PIV3, both of these
viruses protected hamsters completely upon challenge with hPIV3. In
summary, bPIV3 was shown to function as a virus vector that may be
especially suitable for vaccination of infants and children against
PIV3 and other viruses.
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Expression of the Surface Glycoproteins of Human
Parainfluenza Virus Type 3 by Bovine Parainfluenza Virus Type 3, a
Novel Attenuated Virus Vaccine Vector

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Corresponding author. Mailing address: Aviron, 297 N. Bernardo Ave., Mountain View, CA 94043. Phone: (650) 919-1408. Fax: (650) 919-6611. E-mail: ahaller{at}aviron.com.
Present address: Mayo Clinic, Rochester, Minn.
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