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Journal of Virology, December 2000, p. 11464-11471, Vol. 74, No. 24
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Analysis of Individual Human Trigeminal Ganglia for Latent Herpes Simplex Virus Type 1 and Varicella-Zoster Virus Nucleic Acids Using Real-Time PCR

Randall J. Cohrs,1 Jessica Randall,1 John Smith,1 Donald H. Gilden,1,2 Christine Dabrowski,3 Harjeet van der Keyl,3 and Ruth Tal-Singer3,*

Departments of Neurology1 and Microbiology,2 University of Colorado Health Sciences Center, Denver, Colorado 80262, and Department of Molecular Virology and Host Defense, SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania 194263

Received 29 June 2000/Accepted 22 September 2000

Herpes simplex virus type 1 (HSV-1) and varicella-zoster virus (VZV) establish latent infections in the peripheral nervous system following primary infection. During latency both virus genomes exhibit limited transcription, with the HSV-1 LATs and at least four VZV transcripts consistently detected in latently infected human ganglia. In this study we used real-time PCR quantitation to determine the viral DNA copy number in individual trigeminal ganglia (TG) from 17 subjects. The number of HSV-1 genomes was not significantly different between the left and right TG from the same individual and varied per subject from 42.9 to 677.9 copies per 100 ng of DNA. The number of VZV genomes was also not significantly different between left and right TG from the same individual and varied per subject from 37.0 to 3,560.5 copies per 100 ng of DNA. HSV-1 LAT transcripts were consistently detected in ganglia containing latent HSV-1 and varied in relative expression by >500-fold. Of the three VZV transcripts analyzed, only transcripts mapping to gene 63 were consistently detected in latently infected ganglia and varied in relative expression by >2,000-fold. Thus, it appears that, similar to LAT transcription in HSV-1 latently infected ganglia, VZV gene 63 transcription is a hallmark of VZV latency.

* Corresponding author. Mailing address: SmithKline Beecham Pharmaceuticals, 1250 South Collegeville Rd., UP1455, Collegeville, PA 19426. Phone: (610) 917-6869. Fax: (610) 917-4171. E-mail: Ruth_Tal-singer-1{at}sbphrd.com.

Journal of Virology, December 2000, p. 11464-11471, Vol. 74, No. 24
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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