This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, M.-H.
Right arrow Articles by Kao, C. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, M.-H.
Right arrow Articles by Kao, C. C.

 Previous Article  |  Next Article 

Journal of Virology, December 2000, p. 11201-11209, Vol. 74, No. 23
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Efficient and Specific Initiation of Subgenomic RNA Synthesis by Cucumber Mosaic Virus Replicase In Vitro Requires an Upstream RNA Stem-Loop

M.-H. Chen,1 M. J. Roossinck,2 and C. C. Kao1,*

Department of Biology, Indiana University, Bloomington, Indiana 47405,1 and Plant Biology Division, Samuel Robert Noble Foundation, Ardmore, Oklahoma 734022

Received 7 July 2000/Accepted 12 September 2000

We defined the minimal core promoter sequences responsible for efficient and accurate initiation of cucumber mosaic virus (CMV) subgenomic RNA4. The necessary sequence maps to positions -28 to +15 relative to the initiation cytidylate used to initiate RNA synthesis in vivo. Positions -28 to -5 contain a 9-bp stem and a 6-nucleotide purine-rich loop. Considerable changes in the stem and the loop are tolerated for RNA synthesis, including replacement with a different stem-loop. In a template competition assay, the stem-loop and the initiation cytidylate are sufficient to interact with the CMV replicase. Thus, the mechanism of core promoter recognition by the CMV replicase appears to be less specific in comparison to the minimal subgenomic core promoter of the closely related brome mosaic virus.

* Corresponding author. Mailing address: Indiana University, Department of Biology, 1001 E. Third St., Bloomington, IN 47405. Phone: (812) 855-7959. Fax: (812) 855-6705. E-mail: ckao{at}bio.indiana.edu.

Journal of Virology, December 2000, p. 11201-11209, Vol. 74, No. 23
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Thompson, J. R., Buratti, E., de Wispelaere, M., Tepfer, M. (2008). Structural and functional characterization of the 5' region of subgenomic RNA5 of cucumber mosaic virus. J. Gen. Virol. 89: 1729-1738 [Abstract] [Full Text]  
  • Sivakumaran, K., Choi, S.-K., Hema, M., Kao, C. C. (2004). Requirements for Brome Mosaic Virus Subgenomic RNA Synthesis In Vivo and Replicase-Core Promoter Interactions In Vitro. J. Virol. 78: 6091-6101 [Abstract] [Full Text]  
  • van Dijk, A. A., Makeyev, E. V., Bamford, D. H. (2004). Initiation of viral RNA-dependent RNA polymerization. J. Gen. Virol. 85: 1077-1093 [Abstract] [Full Text]  
  • Ayllon, M. A., Gowda, S., Satyanarayana, T., Karasev, A. V., Adkins, S., Mawassi, M., Guerri, J., Moreno, P., Dawson, W. O. (2003). Effects of Modification of the Transcription Initiation Site Context on Citrus Tristeza Virus Subgenomic RNA Synthesis. J. Virol. 77: 9232-9243 [Abstract] [Full Text]  
  • Suzuki, M., Yoshida, M., Yoshinuma, T., Hibi, T. (2003). Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus. J. Gen. Virol. 84: 1931-1939 [Abstract] [Full Text]  
  • Olsthoorn, R. C. L., Bol, J. F. (2002). Role of an Essential Triloop Hairpin and Flanking Structures in the 3' Untranslated Region of Alfalfa Mosaic Virus RNA in In Vitro Transcription. J. Virol. 76: 8747-8756 [Abstract] [Full Text]  
  • Tayon, R. Jr, Kim, M.-J., Kao, C. C. (2001). Completion of RNA synthesis by viral RNA replicases. Nucleic Acids Res 29: 3576-3582 [Abstract] [Full Text]  
  • Wielgosz, M. M., Raju, R., Huang, H. V. (2001). Sequence Requirements for Sindbis Virus Subgenomic mRNA Promoter Function in Cultured Cells. J. Virol. 75: 3509-3519 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»