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Journal of Virology, December 2000, p. 11073-11080, Vol. 74, No. 23
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification and Functional Analysis of the Turnip Yellow Mosaic Tymovirus Subgenomic Promoter

Jan Schirawski,* Ariane Voyatzakis, Bruno Zaccomer,dagger Françoise Bernardi,Dagger and Anne-Lise Haenni

Institut Jacques Monod, 75251 Paris Cedex 05, France

Received 2 May 2000/Accepted 12 September 2000

Most plant viruses rely on the production of subgenomic RNAs (sgRNAs) for the expression of their genes and survival in the plant. Although this is a widely adopted strategy among viruses, the mechanism(s) whereby sgRNA production occurs remains poorly defined. Turnip yellow mosaic tymovirus (TYMV) is a positive-stranded RNA virus that produces an sgRNA for the expression of its coat protein. Here we report that the subgenomic promoter sequence of TYMV is located on a 494-nucleotide fragment, containing previously identified highly conserved sequence elements, which are shown here to be essential for promoter function. After duplication, the subgenomic promoter can be inserted into the coat protein open reading frame, giving rise to the in vivo production of a second sgRNA. It is suggested that this promoter can function when contained on a different molecule than viral genomic RNA. This interesting trait may be of general use for plant and plant virus research.

* Corresponding author. Present address: Department of Microbiology, University College Cork, Western Road, Cork, Ireland. Phone: 353 21 4903686 or 353 21 4902771. Fax: 353 21 4903101. E-mail: schirawski{at}ucc.ie.

dagger Present address: Monsanto, Centre de Recherche de Boissay, Boissay, 28310 Toury, France.

Dagger Present address: Institut de Biotechnologie des Plantes, Université Paris Sud, 91405 Orsay Cedex, France.

Journal of Virology, December 2000, p. 11073-11080, Vol. 74, No. 23
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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