This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rose, N. F.
Right arrow Articles by Rose, J. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rose, N. F.
Right arrow Articles by Rose, J. K.

 Previous Article  |  Next Article 

Journal of Virology, December 2000, p. 10903-10910, Vol. 74, No. 23
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Glycoprotein Exchange Vectors Based on Vesicular Stomatitis Virus Allow Effective Boosting and Generation of Neutralizing Antibodies to a Primary Isolate of Human Immunodeficiency Virus Type 1

Nina F. Rose, Anjeanette Roberts, Linda Buonocore, and John K. Rose*

Departments of Pathology and Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510

Received 21 June 2000/Accepted 31 August 2000

Live recombinant vesicular stomatitis viruses (VSVs) expressing foreign antigens are highly effective vaccine vectors. However, these vectors induce high-titer neutralizing antibody directed at the single VSV glycoprotein (G), and this antibody alone can prevent reinfection and boosting with the same vector. To determine if efficient boosting could be achieved by changing the G protein of the vector, we have developed two new recombinant VSV vectors based on the VSV Indiana serotype but with the G protein gene replaced with G genes from two other VSV serotypes, New Jersey and Chandipura. These G protein exchange vectors grew to titers equivalent to wild-type VSV and induced similar neutralizing titers to themselves but no cross-neutralizing antibodies to the other two serotypes. The effectiveness of these recombinant VSV vectors was illustrated in experiments in which sequential boosting of mice with the three vectors, all encoding the same primary human immunodeficiency virus (HIV) envelope protein, gave a fourfold increase in antibody titer to an oligomeric HIV envelope compared with the response in animals receiving the same vector three times. In addition, only the animals boosted with the exchange vectors produced antibodies neutralizing the autologous HIV primary isolate. These VSV envelope exchange vectors have potential as vaccines in immunizations when boosting of immune responses may be essential.


* Corresponding author. Mailing address: Departments of Pathology and Cell Biology, Yale University School of Medicine, 310 Cedar St., New Haven, CT 06510. Phone: (203) 785-6794. Fax: (203) 785-7467. E-mail: john.rose{at}yale.edu.


Journal of Virology, December 2000, p. 10903-10910, Vol. 74, No. 23
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Wu, K., Kim, G. N., Kang, C. Y. (2009). Expression and processing of human immunodeficiency virus type 1 gp160 using the vesicular stomatitis virus New Jersey serotype vector system. J. Gen. Virol. 90: 1135-1140 [Abstract] [Full Text]  
  • Peng, Y., Lin, F.-c., Verardi, P. H., Jones, L. A., Yilma, T. D. (2009). Lower Levels of Gamma Interferon Expressed by a Pseudotyped Single-Cycle Simian Immunodeficiency Virus Enhance Immunogenicity in Rats. J. Virol. 83: 1592-1601 [Abstract] [Full Text]  
  • Rose, N. F., Publicover, J., Chattopadhyay, A., Rose, J. K. (2008). Hybrid alphavirus-rhabdovirus propagating replicon particles are versatile and potent vaccine vectors. Proc. Natl. Acad. Sci. USA 105: 5839-5843 [Abstract] [Full Text]  
  • Cooper, D., Wright, K. J., Calderon, P. C., Guo, M., Nasar, F., Johnson, J. E., Coleman, J. W., Lee, M., Kotash, C., Yurgelonis, I., Natuk, R. J., Hendry, R. M., Udem, S. A., Clarke, D. K. (2008). Attenuation of Recombinant Vesicular Stomatitis Virus-Human Immunodeficiency Virus Type 1 Vaccine Vectors by Gene Translocations and G Gene Truncation Reduces Neurovirulence and Enhances Immunogenicity in Mice. J. Virol. 82: 207-219 [Abstract] [Full Text]  
  • Peng, Y., Lin, F.-c., Verardi, P. H., Jones, L. A., McChesney, M. B., Yilma, T. D. (2007). Pseudotyped Single-Cycle Simian Immunodeficiency Viruses Expressing Gamma Interferon Augment T-Cell Priming Responses In Vitro. J. Virol. 81: 2187-2195 [Abstract] [Full Text]  
  • Kohl, W., Grone, A., Moennig, V., Herrler, G. (2007). Expression of the surface glycoprotein E2 of Bovine viral diarrhea virus by recombinant vesicular stomatitis virus. J. Gen. Virol. 88: 157-165 [Abstract] [Full Text]  
  • Bukreyev, A., Skiadopoulos, M. H., Murphy, B. R., Collins, P. L. (2006). Nonsegmented negative-strand viruses as vaccine vectors.. J. Virol. 80: 10293-10306 [Full Text]  
  • Publicover, J., Ramsburg, E., Robek, M., Rose, J. K. (2006). Rapid Pathogenesis Induced by a Vesicular Stomatitis Virus Matrix Protein Mutant: Viral Pathogenesis Is Linked to Induction of Tumor Necrosis Factor Alpha. J. Virol. 80: 7028-7036 [Abstract] [Full Text]  
  • Natuk, R. J., Cooper, D., Guo, M., Calderon, P., Wright, K. J., Nasar, F., Witko, S., Pawlyk, D., Lee, M., DeStefano, J., Tummolo, D., Abramovitz, A. S., Gangolli, S., Kalyan, N., Clarke, D. K., Hendry, R. M., Eldridge, J. H., Udem, S. A., Kowalski, J. (2006). Recombinant Vesicular Stomatitis Virus Vectors Expressing Herpes Simplex Virus Type 2 gD Elicit Robust CD4+ Th1 Immune Responses and Are Protective in Mouse and Guinea Pig Models of Vaginal Challenge. J. Virol. 80: 4447-4457 [Abstract] [Full Text]  
  • Potharaju, N R, Potharaju, A K (2006). Is Chandipura virus an emerging human pathogen?. Arch. Dis. Child. 91: 279-280 [Full Text]  
  • Ramsburg, E., Publicover, J., Buonocore, L., Poholek, A., Robek, M., Palin, A., Rose, J. K. (2005). A Vesicular Stomatitis Virus Recombinant Expressing Granulocyte-Macrophage Colony-Stimulating Factor Induces Enhanced T-Cell Responses and Is Highly Attenuated for Replication in Animals. J. Virol. 79: 15043-15053 [Abstract] [Full Text]  
  • Publicover, J., Ramsburg, E., Rose, J. K. (2005). A Single-Cycle Vaccine Vector Based on Vesicular Stomatitis Virus Can Induce Immune Responses Comparable to Those Generated by a Replication-Competent Vector. J. Virol. 79: 13231-13238 [Abstract] [Full Text]  
  • Publicover, J., Ramsburg, E., Rose, J. K. (2004). Characterization of Nonpathogenic, Live, Viral Vaccine Vectors Inducing Potent Cellular Immune Responses. J. Virol. 78: 9317-9324 [Abstract] [Full Text]  
  • Schlehuber, L. D., Rose, J. K. (2004). Prediction and Identification of a Permissive Epitope Insertion Site in the Vesicular Stomatitis Virus Glycoprotein. J. Virol. 78: 5079-5087 [Abstract] [Full Text]  
  • Garbutt, M., Liebscher, R., Wahl-Jensen, V., Jones, S., Moller, P., Wagner, R., Volchkov, V., Klenk, H.-D., Feldmann, H., Stroher, U. (2004). Properties of Replication-Competent Vesicular Stomatitis Virus Vectors Expressing Glycoproteins of Filoviruses and Arenaviruses. J. Virol. 78: 5458-5465 [Abstract] [Full Text]  
  • Ramsburg, E., Rose, N. F., Marx, P. A., Mefford, M., Nixon, D. F., Moretto, W. J., Montefiori, D., Earl, P., Moss, B., Rose, J. K. (2004). Highly Effective Control of an AIDS Virus Challenge in Macaques by Using Vesicular Stomatitis Virus and Modified Vaccinia Virus Ankara Vaccine Vectors in a Single-Boost Protocol. J. Virol. 78: 3930-3940 [Abstract] [Full Text]  
  • Roberts, A., Reuter, J. D., Wilson, J. H., Baldwin, S., Rose, J. K. (2004). Complete Protection from Papillomavirus Challenge after a Single Vaccination with a Vesicular Stomatitis Virus Vector Expressing High Levels of L1 Protein. J. Virol. 78: 3196-3199 [Abstract] [Full Text]  
  • Martinez, I., Rodriguez, L. L., Jimenez, C., Pauszek, S. J., Wertz, G. W. (2003). Vesicular Stomatitis Virus Glycoprotein Is a Determinant of Pathogenesis in Swine, a Natural Host. J. Virol. 77: 8039-8047 [Abstract] [Full Text]  
  • Neumann, G., Whitt, M. A., Kawaoka, Y. (2002). A decade after the generation of a negative-sense RNA virus from cloned cDNA - what have we learned?. J. Gen. Virol. 83: 2635-2662 [Abstract] [Full Text]  
  • Haglund, K., Leiner, I., Kerksiek, K., Buonocore, L., Pamer, E., Rose, J. K. (2002). Robust Recall and Long-Term Memory T-Cell Responses Induced by Prime-Boost Regimens with Heterologous Live Viral Vectors Expressing Human Immunodeficiency Virus Type 1 Gag and Env Proteins. J. Virol. 76: 7506-7517 [Abstract] [Full Text]  
  • Quinones-Kochs, M. I., Buonocore, L., Rose, J. K. (2002). Role of N-Linked Glycans in a Human Immunodeficiency Virus Envelope Glycoprotein: Effects on Protein Function and the Neutralizing Antibody Response. J. Virol. 76: 4199-4211 [Abstract] [Full Text]  
  • Ring, C. J. A. (2002). Cytolytic viruses as potential anti-cancer agents. J. Gen. Virol. 83: 491-502 [Abstract] [Full Text]  
  • Kahn, J. S., Roberts, A., Weibel, C., Buonocore, L., Rose, J. K. (2001). Replication-Competent or Attenuated, Nonpropagating Vesicular Stomatitis Viruses Expressing Respiratory Syncytial Virus (RSV) Antigens Protect Mice against RSV Challenge. J. Virol. 75: 11079-11087 [Abstract] [Full Text]  
  • McGettigan, J. P., Sarma, S., Orenstein, J. M., Pomerantz, R. J., Schnell, M. J. (2001). Expression and Immunogenicity of Human Immunodeficiency Virus Type 1 Gag Expressed by a Replication-Competent Rhabdovirus-Based Vaccine Vector. J. Virol. 75: 8724-8732 [Abstract] [Full Text]