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Journal of Virology, November 2000, p. 10816-10818, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Replication of Wild-Type and Mutant Human Cytomegalovirus in Life-Extended Human Diploid Fibroblasts

Wade A. Bresnahan,* Gretchen E. Hultman,dagger and Thomas Shenk

Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014

Received 7 July 2000/Accepted 21 August 2000

A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.

* Corresponding author. Mailing address: Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014. Phone: (609) 258-5993. Fax: (609) 258-1704. E-mail: wbresnahan{at}molbio.princeton.edu.

dagger Present address: Columbia College of Physicians and Surgeons, New York, NY 10032.

Journal of Virology, November 2000, p. 10816-10818, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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