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Journal of Virology, November 2000, p. 10785-10789, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Thogoto Virus Matrix Protein Is Encoded by a Spliced mRNA

Georg Kochs,* Friedemann Weber,dagger Simone Gruber, Alexander Delvendahl, Caroline Leitz, and Otto Haller

Abteilung für Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Freiburg, D-79008 Freiburg, Germany

Received 22 March 2000/Accepted 15 August 2000

Thogoto virus (THOV) is a tick-transmitted orthomyxovirus with a segmented, negative-stranded RNA genome. In this study, we investigated the coding strategy of RNA segment 6 and found that it contains 956 nucleotides and codes for the matrix (M) protein. The full-length cDNA contains a single, long reading frame that lacks a stop codon but has coding capacity for a putative 35-kDa protein. In contrast, the M protein of THOV has an apparent molecular mass of 29 kDa as assessed by polyacrylamide gel electrophoresis. Therefore, we investigated the possibility of posttranscriptional processing of segment 6 transcripts by reverse transcription-PCR and identified a spliced mRNA that contains a stop codon and is translated into the 29-kDa M protein. Interestingly, the nontemplated UGA stop codon is generated by the splicing event itself. Thus, the unusual M coding strategy of THOV resembles that of Influenza C virus.

* Corresponding author. Mailing address: Abteilung für Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Freiburg, D-79008 Freiburg, Germany. Phone: 49-761-2036623. Fax: 49-761-2036562. E-mail: KOCHS{at}UKL.UNI-FREIBURG.DE.

dagger Present address: Institute of Virology, University of Glasgow, Glasgow G11 5JR, United Kingdom.

Journal of Virology, November 2000, p. 10785-10789, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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