This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leblanc, P.
Right arrow Articles by Vaury, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leblanc, P.
Right arrow Articles by Vaury, C.

 Previous Article  |  Next Article 

Journal of Virology, November 2000, p. 10658-10669, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Life Cycle of an Endogenous Retrovirus, ZAM, in Drosophila melanogaster

P. Leblanc,1 S. Desset,2 F. Giorgi,3 A. R. Taddei,3 A. M. Fausto,3 M. Mazzini,3 B. Dastugue,2 and C. Vaury2,*

ENS/INSERM U 412, 69364 Lyon Cedex 07,1 and Unité INSERM U384, 63000 Clermont-Ferrand,2 France, and Interdepartmental Center for Electron Microscopy, Tuscia University, 00100 Viterbo, Italy3

Received 3 May 2000/Accepted 4 August 2000

ZAM is an env-containing member of the gypsy family of retrotransposons that represents a possible retrovirus of invertebrates. In this paper, we traced ZAM mobilization to get information about a potential path a retroelement may take to reach the germ line of its host. In situ hybridization on whole-mount tissues and immunocytochemistry analyses with antibodies raised against ZAM Gag and Env proteins have shown that all components necessary to assemble ZAM viral particles, i.e., ZAM full-length RNAs and Gag and Env polypeptides, are coexpressed in a small set of follicle cells surrounding the oocyte. By electron microscopy, we have shown that ZAM viral particles are indeed detected in this somatic lineage of cells, which they leave and enter the closely apposed oocyte. Our data provide evidence that the vesicular traffic and yolk granules in the process of vitellogenesis play an important role in ZAM transfer to the oocyte. Our data support the possibility that vitellogenin transfer to the oocyte may help a retroelement pass to the germ line with no need of its envelope product.

* Corresponding author. Mailing address: Unité INSERM U384, BP 38, 28 Place Henri Dunant, 63000 Clermont-Ferrand, France. Phone: (33) 4 73 60 80 24. Fax: (33) 4 73 27 61 32. E-mail: Chantal.VAURY{at}inserm.u-clermont1.fr.

Journal of Virology, November 2000, p. 10658-10669, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Shigenobu, S., Kitadate, Y., Noda, C., Kobayashi, S. (2006). Molecular characterization of embryonic gonads by gene expression profiling in Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 103: 13728-13733 [Abstract] [Full Text]  
  • Havecker, E. R., Gao, X., Voytas, D. F. (2005). The Sireviruses, a Plant-Specific Lineage of the Ty1/copia Retrotransposons, Interact with a Family of Proteins Related to Dynein Light Chain 8. Plant Physiol. 139: 857-868 [Abstract] [Full Text]  
  • Tubio, J. M. C., Naveira, H., Costas, J. (2005). Structural and Evolutionary Analyses of the Ty3/gypsy Group of LTR Retrotransposons in the Genome of Anopheles gambiae. Mol Biol Evol 22: 29-39 [Abstract] [Full Text]  
  • Meignin, C., Dastugue, B., Vaury, C. (2004). Intercellular communication between germ line and somatic line is utilized to control the transcription of ZAM, an endogenous retrovirus from Drosophila melanogaster. Nucleic Acids Res 32: 3799-3806 [Abstract] [Full Text]  
  • Desset, S., Meignin, C., Dastugue, B., Vaury, C. (2003). COM, a Heterochromatic Locus Governing the Control of Independent Endogenous Retroviruses From Drosophila melanogaster. Genetics 164: 501-509 [Abstract] [Full Text]  
  • Teysset, L., Dang, V.-D., Kim, M. K., Levin, H. L. (2003). A Long Terminal Repeat-Containing Retrotransposon of Schizosaccharomyces pombe Expresses a Gag-Like Protein That Assembles into Virus-Like Particles Which Mediate Reverse Transcription. J. Virol. 77: 5451-5463 [Abstract] [Full Text]  
  • Conte, C., Dastugue, B., Vaury, C. (2002). Coupling of Enhancer and Insulator Properties Identified in Two Retrotransposons Modulates Their Mutagenic Impact on Nearby Genes. Mol. Cell. Biol. 22: 1767-1777 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»