This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Soudais, C.
Right arrow Articles by Kremer, E. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Soudais, C.
Right arrow Articles by Kremer, E. J.

 Previous Article  |  Next Article 

Journal of Virology, November 2000, p. 10639-10649, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Canine Adenovirus Type 2 Attachment and Internalization: Coxsackievirus-Adenovirus Receptor, Alternative Receptors, and an RGD-Independent Pathway

Claire Soudais,1 Sylvie Boutin,1 Saw See Hong,2 Miguel Chillon,1 Olivier Danos,1 Jeffrey M. Bergelson,3 Pierre Boulanger,2 and Eric J. Kremer1,*

Généthon III and CNRS URA 1923, Evry,1 and Laboratoire de Virologie & Pathogénèse Virale, CNRS UMR 5537, Lyon,2 France, and Division of Immunologic and Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania3

Received 1 May 2000/Accepted 21 August 2000

The best-characterized receptors for adenoviruses (Ads) are the coxsackievirus-Ad receptor (CAR) and integrins alpha vbeta 5 and alpha vbeta 3, which facilitate entry. The alpha v integrins recognize an Arg-Gly-Asp (RGD) motif found in some extracellular matrix proteins and in the penton base in most human Ads. Using a canine adenovirus type 2 (CAV-2) vector, we found that CHO cells that express CAR but not wild-type CHO cells are susceptible to CAV-2 transduction. Cells expressing alpha Mbeta 2 integrins or major histocompatibility complex class I (MHC-I) molecules but which do not express CAR were not transduced. Binding assays showed that CAV-2 attaches to a recombinant soluble form of CAR and that Ad type 5 (Ad5) fiber, penton base, and an anti-CAR antibody partially blocked attachment. Using fluorescently labeled CAV-2 particles, we found that in some cells nonpermissive for transduction, inhibition was at the point of internalization and not attachment. The transduction efficiency of CAV-2, which lacks an RGD motif, surprisingly mimicked that of Ad5 when tested in cells selectively expressing alpha vbeta 5 and alpha vbeta 3 integrins. Our results demonstrate that CAV-2 transduction is augmented by CAR and possibly by alpha vbeta 5, though transduction can be CAR and alpha vbeta 3/5 independent but is alpha Mbeta 2, MHC-I, and RGD independent, demonstrating a transduction mechanism which is distinct from that of Ad2/5.

* Corresponding author. Mailing address: Généthon III/CNRS URA 1923, 1bis, rue de l'Internationale, 91002 Evry, France. Phone: 33 (0) 1-69-47-10-30. Fax: 33 (0) 1-69-47-28-38. E-mail: ekremer{at}genethon.fr.

Journal of Virology, November 2000, p. 10639-10649, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Szelechowski, M., Fournier, A., Richardson, J., Eloit, M., Klonjkowski, B. (2009). Functional organization of the major late transcriptional unit of canine adenovirus type 2. J. Gen. Virol. 90: 1215-1223 [Abstract] [Full Text]  
  • Schoehn, G., El Bakkouri, M., Fabry, C. M. S., Billet, O., Estrozi, L. F., Le, L., Curiel, D. T., Kajava, A. V., Ruigrok, R. W. H., Kremer, E. J. (2008). Three-Dimensional Structure of Canine Adenovirus Serotype 2 Capsid. J. Virol. 82: 3192-3203 [Abstract] [Full Text]  
  • Hassan, M.H., Khatoon, N., Curiel, D.T., Hamada, F.M., Arafa, H.M., Al-Hendy, A. (2008). Toward gene therapy of uterine fibroids: targeting modified adenovirus to human leiomyoma cells. Hum Reprod 23: 514-524 [Abstract] [Full Text]  
  • Perreau, M., Mennechet, F., Serratrice, N., Glasgow, J. N., Curiel, D. T., Wodrich, H., Kremer, E. J. (2007). Contrasting Effects of Human, Canine, and Hybrid Adenovirus Vectors on the Phenotypical and Functional Maturation of Human Dendritic Cells: Implications for Clinical Efficacy. J. Virol. 81: 3272-3284 [Abstract] [Full Text]  
  • Seiradake, E., Lortat-Jacob, H., Billet, O., Kremer, E. J., Cusack, S. (2006). Structural and Mutational Analysis of Human Ad37 and Canine Adenovirus 2 Fiber Heads in Complex with the D1 Domain of Coxsackie and Adenovirus Receptor. J. Biol. Chem. 281: 33704-33716 [Abstract] [Full Text]  
  • Keriel, A., Rene, C., Galer, C., Zabner, J., Kremer, E. J. (2006). Canine Adenovirus Vectors for Lung-Directed Gene Transfer: Efficacy, Immune Response, and Duration of Transgene Expression Using Helper-Dependent Vectors. J. Virol. 80: 1487-1496 [Abstract] [Full Text]  
  • Perreau, M., Kremer, E. J. (2005). Frequency, Proliferation, and Activation of Human Memory T Cells Induced by a Nonhuman Adenovirus. J. Virol. 79: 14595-14605 [Abstract] [Full Text]  
  • Zhang, Y., Bergelson, J. M. (2005). Adenovirus Receptors. J. Virol. 79: 12125-12131 [Full Text]  
  • Skog, J., Edlund, K., Widegren, B., Salford, L. G., Wadell, G., Mei, Y.-F. (2004). Efficient internalization into low-passage glioma cell lines using adenoviruses other than type 5: an approach for improvement of gene delivery to brain tumours. J. Gen. Virol. 85: 2627-2638 [Abstract] [Full Text]  
  • Shayakhmetov, D. M., Li, Z.-Y., Ternovoi, V., Gaggar, A., Gharwan, H., Lieber, A. (2003). The Interaction between the Fiber Knob Domain and the Cellular Attachment Receptor Determines the Intracellular Trafficking Route of Adenoviruses. J. Virol. 77: 3712-3723 [Abstract] [Full Text]  
  • Petrella, J., Cohen, C. J., Gaetz, J., Bergelson, J. M. (2002). A Zebrafish Coxsackievirus and Adenovirus Receptor Homologue Interacts with Coxsackie B Virus and Adenovirus. J. Virol. 76: 10503-10506 [Abstract] [Full Text]  
  • Cohen, C. J., Xiang, Z. Q., Gao, G.-P., Ertl, H. C. J., Wilson, J. M., Bergelson, J. M. (2002). Chimpanzee adenovirus CV-68 adapted as a gene delivery vector interacts with the coxsackievirus and adenovirus receptor. J. Gen. Virol. 83: 151-155 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»