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Journal of Virology, November 2000, p. 10581-10588, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of Three nef-Defective Human Immunodeficiency Virus Type 1 Strains Associated with Long-Term Nonprogression

David I. Rhodes,1,* Lesley Ashton,2 Ajantha Solomon,1 Andrew Carr,3 David Cooper,2,3 John Kaldor,2 and Nicholas Deacondagger for the Australian Long-Term Nonprogressor Study Group

AIDS Molecular Biology Unit, National Centre in HIV Virology Research, Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria 3078,1 and National Centre in HIV Epidemiology and Clinical Research, University of New South Wales,2 and HIV Medicine Unit and Centre for Immunology, St. Vincent's Hospital,3 Sydney, New South Wales 2010, Australia

Received 23 December 1999/Accepted 23 August 2000

Long-term survivors (LTS) of human immunodeficiency virus type 1 (HIV-1) infection provide an opportunity to investigate both viral and host factors that influence the rate of disease progression. We have identified three HIV-1-infected individuals in Australia who have been infected for over 11 years with viruses that contain deletions in the nef and nef-long terminal repeat (nef/LTR) overlap regions. These viruses differ from each other and from other nef-defective strains of HIV-1 previously identified in Australia. One individual, LTS 3, is infected with a virus containing a nef gene with a deletion of 29 bp from the nef/LTR overlap region, resulting in a truncated Nef open reading frame. In addition to the Nef defect, only viruses containing truncated Vif open reading frames of 37 or 69 amino acids could be detected in peripheral blood mononuclear cells isolated from this patient. LTS 3 had a viral load of less than 20 copies of RNA/ml of plasma. The other two long-term survivors, LTS 9 and LTS 11, had loads of less than 200 copies of RNA/ml of plasma and are infected with viruses with larger deletions in both the nef alone and nef/LTR overlap regions. These viruses contain wild-type vif, vpu, and vpr accessory genes. All three strains of virus had envelope sequences characteristic of macrophagetropic viruses. These findings further indicate the reduced pathogenic potential of nef-defective viruses.

* Corresponding author. Mailing address: AMRAD Operations, 576 Swan St., Richmond, Victoria 3121, Australia. Phone: 61-3-9208 4181. Fax: 61-3-9208 4100. E-mail: drhodes{at}amrad.com.au.

dagger The Australian Long-Term Nonprogressor Study Group includes B. Anderson, A. Allworth, L. Ashton, D. Baker, G. Batty, R. Biti, M. Bloch, N. Bodsworth, C. Bourne, J. Byrne, A. Carr, K. Clare, D. Cooper, E. Cummins, P. Cunningham, D. Couldwell, L. Dayan, N. Deacon, A. Dearden, N. Doong, F. Drummond, J. Ewan, R. Finlayson, R. Ffrench, V. Furner, B. Genn, R. Hain, J. Kaldor, J. Kidd, M. Law, A. Lloyd, R. McFarlane, D. McPhee, H. Michelmore, K. McGhie, M. McMurchie, K. Mutimer, C. Pell, J. Peterson, R. Price, D. Quan, J. Quin, H. Ree, D. Rhodes, M. Robertson, C. Satchell, D. Smith, A. Solomon, D. Spencer, G. Stewart, J. Sullivan, and J. Ziegler.

Journal of Virology, November 2000, p. 10581-10588, Vol. 74, No. 22
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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