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Journal of Virology, November 2000, p. 9964-9971, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Cells Expressing the Epstein-Barr Virus Growth
Program Are Present in and Restricted to the Naive B-Cell Subset of
Healthy Tonsils
Alexandra M.
Joseph,
Gregory
J.
Babcock, and
David A.
Thorley-Lawson*
Department of Pathology, Tufts University
School of Medicine, Boston, Massachusetts 02111
Received 5 May 2000/Accepted 8 August 2000
In this paper we demonstrate, for the first time, that Epstein-Barr
virus (EBV)-infected cells expressing the lymphoblastoid growth program
are present in healthy carriers of the virus. Previously we observed
that latently infected naive B cells are present in tonsils only when
viral replication is detected, suggesting that these may represent
newly infected B cells. We have tested this idea by performing a
reverse transcription-PCR analysis for the expression of latent genes
(EBNA2 and the EBNA3s) that are characteristically expressed only by
newly infected cells expressing the growth latency program. EBNA2
expression is regularly detected in purified naive (IgD+)
tonsillar B cells (13 of 16 tonsils tested) but was never found in the
IgD
population (0 of 16). More detailed analysis revealed
that the mRNAs for the latent genes EBNA1 (3 of 3 tonsils tested),
EBNA3a (3 of 5), EBNA3b (3 of 5), EBNA3c (3 of 5), LMP1 (6 of 6), and LMP2 (5 of 6) were also present in the IgD+ population, but
the EBNA1Q-K transcript, characteristic of nonlymphoblastoid forms of
latency, was never detected (0 of 6). Finally, we demonstrate that the
latently infected naive (IgD+) cells express CD80 (B7.1), a
marker characteristically expressed on activated naive lymphoblasts but
absent from resting naive B cells. The infected naive
(IgD+) population in the tonsil therefore has the viral and
cellular phenotype of a B-cell directly infected with EBV
an activated lymphoblast expressing the growth program.
*
Corresponding author. Mailing address: Dept. of
Pathology, Tufts University School of Medicine, 136 Harrison Ave.,
Boston, MA 02111. Phone: (617) 636-2726. Fax: (617) 636-2990. E-mail: dlawson{at}opal.tufts.edu.
Journal of Virology, November 2000, p. 9964-9971, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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