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Journal of Virology, November 2000, p. 9911-9915, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Measles Virus Assembly within Membrane Rafts

Séverine Vincent, Denis Gerlier,* and Serge N. Manié

Immunité & Infections Virales, VPV, CNRS-UCBL UMR 5537, Faculté de Médecine Lyon RTH Laennec, 69372 Lyon Cedex 08, France

Received 29 February 2000/Accepted 10 August 2000

During measles virus (MV) replication, approximately half of the internal M and N proteins, together with envelope H and F glycoproteins, are selectively enriched in microdomains rich in cholesterol and sphingolipids called membrane rafts. Rafts isolated from MV-infected cells after cold Triton X-100 solubilization and flotation in a sucrose gradient contain all MV components and are infectious. Furthermore, the H and F glycoproteins from released virus are also partly in membrane rafts (S. N. Manié et al., J. Virol. 74:305-311, 2000). When expressed alone, the M but not N protein shows a low partitioning (around 10%) into rafts; this distribution is unchanged when all of the internal proteins, M, N, P, and L, are coexpressed. After infection with MGV, a chimeric MV where both H and F proteins have been replaced by vesicular stomatitis virus G protein, both the M and N proteins were found enriched in membrane rafts, whereas the G protein was not. These data suggest that assembly of internal MV proteins into rafts requires the presence of the MV genome. The F but not H glycoprotein has the intrinsic ability to be localized in rafts. When coexpressed with F, the H glycoprotein is dragged into the rafts. This is not observed following coexpression of either the M or N protein. We propose a model for MV assembly into membrane rafts where the virus envelope and the ribonucleoparticle colocalize and associate.

* Corresponding author. Mailing address: Immunité & Infections Virales, VPV, CNRS-UCBL UMR 5537, Faculté de Médecine Lyon RTH Laennec, 69372 Lyon Cedex 08, France. Phone: 33 4 78 77 86 18. Fax: 33 4 78 77 87 54. E-mail: gerlier{at}laennec.univ-lyon1.fr.

Journal of Virology, November 2000, p. 9911-9915, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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