This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jacob, Y.
Right arrow Articles by Tordo, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jacob, Y.
Right arrow Articles by Tordo, N.

 Previous Article  |  Next Article 

Journal of Virology, November 2000, p. 10217-10222, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cytoplasmic Dynein LC8 Interacts with Lyssavirus Phosphoprotein

Yves Jacob,1,* Hassan Badrane,1 Pierre-Emmanuel Ceccaldi,2 and Noël Tordo1

Laboratoire des Lyssavirus1 and Unité de la Rage,2 Institut Pasteur, 75724 Paris Cedex 15, France

Received 3 April 2000/Accepted 26 July 2000

Using a yeast two-hybrid human brain cDNA library screen, the cytoplasmic dynein light chain (LC8), a 10-kDa protein, was found to interact strongly with the phosphoprotein (P) of two lyssaviruses: rabies virus (genotype 1) and Mokola virus (genotype 3). The high degree of sequence divergence between these P proteins (only 46% amino acid identity) favors the hypothesis that this interaction is a common property shared by all lyssaviruses. The P protein-dynein LC8 interaction was confirmed by colocalization with laser confocal microscopy in infected cells and by coimmunoprecipitation. The dynein-interacting P protein domain was mapped to the 186 amino acid residues of the N-terminal half of the protein. Dynein LC8 is a component of both cytoplasmic dynein and myosin V, which are involved in a wide range of intracellular motile events, such as microtubule minus-end directed organelle transport in axon "retrograde transport" and actin-based vesicle transport, respectively. Our results provide support for a model of viral nucleocapsid axoplasmic transport. Furthermore, the role of LC8 in cellular mechanisms other than transport, e.g., inhibition of neuronal nitric oxide synthase, suggests that the P protein interactions could be involved in physiopathological mechanisms of rabies virus-induced pathogenesis.

* Corresponding author. Mailing address: Laboratoire des Lyssavirus, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France. Phone: 33 (0) 1 45 68 87 53. Fax: 33 (0) 1 40 61 32 56. E-mail: yjacob{at}pasteur.fr.

Journal of Virology, November 2000, p. 10217-10222, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lahaye, X., Vidy, A., Pomier, C., Obiang, L., Harper, F., Gaudin, Y., Blondel, D. (2009). Functional Characterization of Negri Bodies (NBs) in Rabies Virus-Infected Cells: Evidence that NBs Are Sites of Viral Transcription and Replication. J. Virol. 83: 7948-7958 [Abstract] [Full Text]  
  • Kubota, T., Matsuoka, M., Chang, T.-H., Bray, M., Jones, S., Tashiro, M., Kato, A., Ozato, K. (2009). Ebolavirus VP35 Interacts with the Cytoplasmic Dynein Light Chain 8. J. Virol. 83: 6952-6956 [Abstract] [Full Text]  
  • Song, C., Wen, W., Rayala, S. K., Chen, M., Ma, J., Zhang, M., Kumar, R. (2008). Serine 88 Phosphorylation of the 8-kDa Dynein Light Chain 1 Is a Molecular Switch for Its Dimerization Status and Functions. J. Biol. Chem. 283: 4004-4013 [Abstract] [Full Text]  
  • Klingen, Y., Conzelmann, K.-K., Finke, S. (2008). Double-Labeled Rabies Virus: Live Tracking of Enveloped Virus Transport. J. Virol. 82: 237-245 [Abstract] [Full Text]  
  • Moseley, G. W., Roth, D. M., DeJesus, M. A., Leyton, D. L., Filmer, R. P., Pouton, C. W., Jans, D. A. (2007). Dynein Light Chain Association Sequences Can Facilitate Nuclear Protein Import. Mol. Biol. Cell 18: 3204-3213 [Abstract] [Full Text]  
  • Tan, G. S., Preuss, M. A. R., Williams, J. C., Schnell, M. J. (2007). The dynein light chain 8 binding motif of rabies virus phosphoprotein promotes efficient viral transcription. Proc. Natl. Acad. Sci. USA 104: 7229-7234 [Abstract] [Full Text]  
  • Das, S. C., Nayak, D., Zhou, Y., Pattnaik, A. K. (2006). Visualization of intracellular transport of vesicular stomatitis virus nucleocapsids in living cells.. J. Virol. 80: 6368-6377 [Abstract] [Full Text]  
  • Vidy, A., Chelbi-Alix, M., Blondel, D. (2005). Rabies Virus P Protein Interacts with STAT1 and Inhibits Interferon Signal Transduction Pathways. J. Virol. 79: 14411-14420 [Abstract] [Full Text]  
  • Wang, Z. W., Sarmento, L., Wang, Y., Li, X.-q., Dhingra, V., Tseggai, T., Jiang, B., Fu, Z. F. (2005). Attenuated Rabies Virus Activates, while Pathogenic Rabies Virus Evades, the Host Innate Immune Responses in the Central Nervous System. J. Virol. 79: 12554-12565 [Abstract] [Full Text]  
  • Havecker, E. R., Gao, X., Voytas, D. F. (2005). The Sireviruses, a Plant-Specific Lineage of the Ty1/copia Retrotransposons, Interact with a Family of Proteins Related to Dynein Light Chain 8. Plant Physiol. 139: 857-868 [Abstract] [Full Text]  
  • Brzozka, K., Finke, S., Conzelmann, K.-K. (2005). Identification of the Rabies Virus Alpha/Beta Interferon Antagonist: Phosphoprotein P Interferes with Phosphorylation of Interferon Regulatory Factor 3. J. Virol. 79: 7673-7681 [Abstract] [Full Text]  
  • Williams, J. C., Xie, H., Hendrickson, W. A. (2005). Crystal Structure of Dynein Light Chain TcTex-1. J. Biol. Chem. 280: 21981-21986 [Abstract] [Full Text]  
  • Strunze, S., Trotman, L. C., Boucke, K., Greber, U. F. (2005). Nuclear Targeting of Adenovirus Type 2 Requires CRM1-mediated Nuclear Export. Mol. Biol. Cell 16: 2999-3009 [Abstract] [Full Text]  
  • Lo, K. W.-H., Kan, H.-M., Chan, L.-N., Xu, W.-G., Wang, K.-P., Wu, Z., Sheng, M., Zhang, M. (2005). The 8-kDa Dynein Light Chain Binds to p53-binding Protein 1 and Mediates DNA Damage-induced p53 Nuclear Accumulation. J. Biol. Chem. 280: 8172-8179 [Abstract] [Full Text]  
  • Finke, S., Brzozka, K., Conzelmann, K.-K. (2004). Tracking Fluorescence-Labeled Rabies Virus: Enhanced Green Fluorescent Protein-Tagged Phosphoprotein P Supports Virus Gene Expression and Formation of Infectious Particles. J. Virol. 78: 12333-12343 [Abstract] [Full Text]  
  • Kelkar, S. A., Pfister, K. K., Crystal, R. G., Leopold, P. L. (2004). Cytoplasmic Dynein Mediates Adenovirus Binding to Microtubules. J. Virol. 78: 10122-10132 [Abstract] [Full Text]  
  • Real, E., Rain, J.-C., Battaglia, V., Jallet, C., Perrin, P., Tordo, N., Chrisment, P., D'Alayer, J., Legrain, P., Jacob, Y. (2004). Antiviral Drug Discovery Strategy Using Combinatorial Libraries of Structurally Constrained Peptides. J. Virol. 78: 7410-7417 [Abstract] [Full Text]  
  • Douglas, M. W., Diefenbach, R. J., Homa, F. L., Miranda-Saksena, M., Rixon, F. J., Vittone, V., Byth, K., Cunningham, A. L. (2004). Herpes Simplex Virus Type 1 Capsid Protein VP26 Interacts with Dynein Light Chains RP3 and Tctex1 and Plays a Role in Retrograde Cellular Transport. J. Biol. Chem. 279: 28522-28530 [Abstract] [Full Text]  
  • Petit, C., Giron, M.-L., Tobaly-Tapiero, J., Bittoun, P., Real, E., Jacob, Y., Tordo, N., de The, H., Saib, A. (2003). Targeting of incoming retroviral Gag to the centrosome involves a direct interaction with the dynein light chain 8. J. Cell Sci. 116: 3433-3442 [Abstract] [Full Text]  
  • Kaiser, F. J., Tavassoli, K., Van den Bemd, G.-J., Chang, G. T.G., Horsthemke, B., Moroy, T., Ludecke, H.-J. (2003). Nuclear interaction of the dynein light chain LC8a with the TRPS1 transcription factor suppresses the transcriptional repression activity of TRPS1. Hum Mol Genet 12: 1349-1358 [Abstract] [Full Text]  
  • McGettigan, J. P., Pomerantz, R. J., Siler, C. A., McKenna, P. M., Foley, H. D., Dietzschold, B., Schnell, M. J. (2002). Second-Generation Rabies Virus-Based Vaccine Vectors Expressing Human Immunodeficiency Virus Type 1 Gag Have Greatly Reduced Pathogenicity but Are Highly Immunogenic. J. Virol. 77: 237-244 [Abstract] [Full Text]  
  • Toriumi, H., Honda, Y., Morimoto, K., Tochikura, T. S., Kawai, A. (2002). Structural relationship between nucleocapsid-binding activity of the rabies virus phosphoprotein (P) and exposure of epitope 402-13 located at the C terminus. J. Gen. Virol. 83: 3035-3043 [Abstract] [Full Text]  
  • Mueller, S., Cao, X., Welker, R., Wimmer, E. (2002). Interaction of the Poliovirus Receptor CD155 with the Dynein Light Chain Tctex-1 and Its Implication for Poliovirus Pathogenesis. J. Biol. Chem. 277: 7897-7904 [Abstract] [Full Text]  
  • Mebatsion, T. (2001). Extensive Attenuation of Rabies Virus by Simultaneously Modifying the Dynein Light Chain Binding Site in the P Protein and Replacing Arg333 in the G Protein. J. Virol. 75: 11496-11502 [Abstract] [Full Text]  
  • Poisson, N., Real, E., Gaudin, Y., Vaney, M.-C., King, S., Jacob, Y., Tordo, N., Blondel, D. (2001). Molecular basis for the interaction between rabies virus phosphoprotein P and the dynein light chain LC8: dissociation of dynein-binding properties and transcriptional functionality of P. J. Gen. Virol. 82: 2691-2696 [Abstract] [Full Text]  
  • Jacob, Y., Real, E., Tordo, N. (2001). Functional Interaction Map of Lyssavirus Phosphoprotein: Identification of the Minimal Transcription Domains. J. Virol. 75: 9613-9622 [Abstract] [Full Text]  
  • Alonso, C., Miskin, J., Hernaez, B., Fernandez-Zapatero, P., Soto, L., Canto, C., Rodriguez-Crespo, I., Dixon, L., Escribano, J. M. (2001). African Swine Fever Virus Protein p54 Interacts with the Microtubular Motor Complex through Direct Binding to Light-Chain Dynein. J. Virol. 75: 9819-9827 [Abstract] [Full Text]  
  • Raux, H., Flamand, A., Blondel, D. (2000). Interaction of the Rabies Virus P Protein with the LC8 Dynein Light Chain. J. Virol. 74: 10212-10216 [Abstract] [Full Text]  
  • Lo, K. W.-H., Naisbitt, S., Fan, J.-S., Sheng, M., Zhang, M. (2001). The 8-kDa Dynein Light Chain Binds to Its Targets via a Conserved (K/R)XTQT Motif. J. Biol. Chem. 276: 14059-14066 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»