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Journal of Virology, November 2000, p. 10202-10206, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Restricting Expression Prolongs Expression of Foreign Genes Introduced into Animals by Retroviruses

Valerian B. Pinto,1,dagger Shiv Prasad,2,Dagger Jonathan Yewdell,2 Jack Bennink,2 and Stephen H. Hughes1,*

ABL-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201,1 and Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 208922

Received 17 May 2000/Accepted 24 July 2000

If foreign genes are ubiquitously expressed in mice using a viral vector, expression is abrogated by CD8+ cells in 2 to 4 weeks. However, if the expression of the genes is confined to skeletal muscle cells, the CD8+ T-cell response is much weaker and expression is maintained for more than 6 weeks. These data show that restricting the expression of foreign genes to skeletal muscle cells and presumably to other cells that are inefficient at antigen presentation can prolong the expression of a foreign gene product.

* Corresponding author. Mailing address: NCI-Frederick Cancer Research and Development Center, P.O. Box B, Bldg. 539, Frederick, MD 21702-1201. Phone: (301) 846-1619. Fax: (301) 846-6966. E-mail: hughes{at}ncifcrf.gov.

dagger Present address: HIV Drug Resistance Program, National Cancer Institute-FCRDC, Frederick, MD 21702-1201.

Dagger Present address: Division of Allergy, Immunology, and Transplantation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

Journal of Virology, November 2000, p. 10202-10206, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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