This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, X.-P.
Right arrow Articles by Fink, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, X.-P.
Right arrow Articles by Fink, D. J.

 Previous Article  |  Next Article 

Journal of Virology, November 2000, p. 10132-10141, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Herpes Simplex Virus Type 1 ICP0 Protein Does Not Accumulate in the Nucleus of Primary Neurons in Culture

Xiao-Ping Chen,1 Jia Li,1 Marina Mata,1,2 James Goss,1 Darren Wolfe,3 Joseph C. Glorioso,3 and David J. Fink1,2,3,*

Departments of Neurology1 and Molecular Genetics and Biochemistry,3 University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, and VA Medical Center, Pittsburgh, Pennsylvania 152402

Received 2 May 2000/Accepted 9 August 2000

Infected-cell protein 0 (ICP0), the product of the herpes simplex virus (HSV) immediate-early (IE) alpha 0 gene, is a promiscuous transactivator of viral early (E) and late (L) gene expression. HSV mutants lacking ICP0 function are severely deficient in viral growth and protein synthesis, particularly at low multiplicities of infection. Early in the infectious process in vitro, ICP0 protein accumulates in distinct domains within the nucleus to form characteristic structures active in the transcription of viral genes. However, following infection of primary trigeminal ganglion cells in vitro with a recombinant HSV mutant that expresses only ICP0, we observed that ICP0 protein accumulated in the characteristic intranuclear distribution only in the nuclei of Schwann cells; neurons in the culture did not accumulate ICP0 despite expression of ICP0 RNA in those cells. The same phenomenon was observed in PC12 cells differentiated to assume a neuronal phenotype. In primary neurons in culture, the amount of ICP0 protein could be increased by pharmacologic inhibition of calcium-activated protease (calpain) activity or by inhibition of protein phosphatase 2B (calcineurin). The failure of ICP0 protein to accumulate in the nucleus of neurons suggests that one mechanism which may impair efficient replication of the virus in neurons, and thus favor the establishment of viral latency in those cells, may be found in the cell-specific processing of that IE gene product.

* Corresponding author. Mailing address: S-520 BST, 200 Lothrop St., Pittsburgh, PA 15213. Phone: (412) 648-9793. Fax: (412) 648-8081. E-mail: dfink{at}med.pitt.edu.

Journal of Virology, November 2000, p. 10132-10141, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Terry-Allison, T., Smith, C. A., DeLuca, N. A. (2007). Relaxed Repression of Herpes Simplex Virus Type 1 Genomes in Murine Trigeminal Neurons. J. Virol. 81: 12394-12405 [Abstract] [Full Text]  
  • Frampton, A. R. Jr., Stolz, D. B., Uchida, H., Goins, W. F., Cohen, J. B., Glorioso, J. C. (2007). Equine Herpesvirus 1 Enters Cells by Two Different Pathways, and Infection Requires the Activation of the Cellular Kinase ROCK1. J. Virol. 81: 10879-10889 [Abstract] [Full Text]  
  • Morishige, N., Jester, J. V., Naito, J., Osorio, N., Wahlert, A., Jones, C., Everett, R. D., Wechsler, S. L., Perng, G. C. (2006). Herpes simplex virus type 1 ICP0 localizes in the stromal layer of infected rabbit corneas and resides predominantly in the cytoplasm and/or perinuclear region of rabbit keratocytes.. J. Gen. Virol. 87: 2817-2825 [Abstract] [Full Text]  
  • Kwon, H., Bai, Q., Baek, H.-J., Felmet, K., Burton, E. A., Goins, W. F., Cohen, J. B., Glorioso, J. C. (2006). Soluble V Domain of Nectin-1/HveC Enables Entry of Herpes Simplex Virus Type 1 (HSV-1) into HSV-Resistant Cells by Binding to Viral Glycoprotein D. J. Virol. 80: 138-148 [Abstract] [Full Text]  
  • Frampton, A. R. Jr., Goins, W. F., Cohen, J. B., von Einem, J., Osterrieder, N., O'Callaghan, D. J., Glorioso, J. C. (2005). Equine Herpesvirus 1 Utilizes a Novel Herpesvirus Entry Receptor. J. Virol. 79: 3169-3173 [Abstract] [Full Text]  
  • Jiang, C., Wechuck, J. B., Goins, W. F., Krisky, D. M., Wolfe, D., Ataai, M. M., Glorioso, J. C. (2004). Immobilized Cobalt Affinity Chromatography Provides a Novel, Efficient Method for Herpes Simplex Virus Type 1 Gene Vector Purification. J. Virol. 78: 8994-9006 [Abstract] [Full Text]  
  • Jackson, S. A., DeLuca, N. A. (2003). Relationship of herpes simplex virus genome configuration to productive and persistent infections. Proc. Natl. Acad. Sci. USA 100: 7871-7876 [Abstract] [Full Text]  
  • Burton, E. A., Hong, C.-S., Glorioso, J. C. (2003). The Stable 2.0-Kilobase Intron of the Herpes Simplex Virus Type 1 Latency-Associated Transcript Does Not Function as an Antisense Repressor of ICP0 in Nonneuronal Cells. J. Virol. 77: 3516-3530 [Abstract] [Full Text]  
  • Jones, C. (2003). Herpes Simplex Virus Type 1 and Bovine Herpesvirus 1 Latency. Clin. Microbiol. Rev. 16: 79-95 [Abstract] [Full Text]  
  • Luciano, R. L., Wilson, A. C. (2002). An activation domain in the C-terminal subunit of HCF-1 is important for transactivation by VP16 and LZIP. Proc. Natl. Acad. Sci. USA 99: 13403-13408 [Abstract] [Full Text]  
  • Arthur, J. L., Scarpini, C. G., Connor, V., Lachmann, R. H., Tolkovsky, A. M., Efstathiou, S. (2001). Herpes Simplex Virus Type 1 Promoter Activity during Latency Establishment, Maintenance, and Reactivation in Primary Dorsal Root Neurons In Vitro. J. Virol. 75: 3885-3895 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»