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Journal of Virology, November 2000, p. 10104-10111, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Interaction of Epstein-Barr Virus Nuclear Antigen
Leader Protein (EBNA-LP) with HS1-Associated Protein X-1: Implication
of Cytoplasmic Function of EBNA-LP
Yasushi
Kawaguchi,
Kaori
Nakajima,
Mie
Igarashi,
Tomoko
Morita,
Michiko
Tanaka,
Mikiko
Suzuki,
Akihiko
Yokoyama,
Go
Matsuda,
Kentaro
Kato,
Mikiko
Kanamori, and
Kanji
Hirai*
Department of Tumor Virology, Division of
Virology and Immunology, Medical Research Institute, Tokyo Medical
and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan
Received 12 June 2000/Accepted 11 August 2000
Epstein-Barr virus (EBV) nuclear antigen leader protein (EBNA-LP)
consists of W1W2 repeats and a unique C-terminal Y1Y2 domain and has
been suggested to play an important role in EBV-induced transformation.
To identify the cellular factors interacting with EBNA-LP, we performed
a yeast two-hybrid screen, using EBNA-LP cDNA containing four W1W2
repeats as bait and an EBV-transformed human peripheral blood
lymphocyte cDNA library as the source of cellular genes. Our results
were as follows. (i) All three cDNAs in positive yeast colonies were
found to encode the same cellular protein, HS1-associated protein X-1
(HAX-1), which is localized mainly in the cytoplasm and has been
suggested to be involved in the regulation of B-cell signal
transduction and apoptosis. (ii) Mutational analysis of EBNA-LP
revealed that the association with HAX-1 is mediated by the W1W2 repeat
domain. (iii) A purified chimeric protein consisting of glutathione
S-transferase fused to EBNA-LP specifically formed
complexes with HAX-1 transiently expressed in COS-7 cells. (iv) When
EBNA-LP and HAX-1 were coexpressed in COS-7 cells, EBNA-LP was
specifically coimmunoprecipitated with HAX-1. (v) Careful cell
fractionation experiments of an EBV-infected lymphoblastoid cell line
revealed that EBNA-LP is localized in the cytoplasm as well as in the
nucleus. (vi) When EBNA-LP containing four W1W2 repeats was expressed
in COS-7 cells, EBNA-LP was detected mainly in the nucleus by
immunofluorescence assay. Interestingly, when EBNA-LP containing a
single W1W2 repeat was expressed in COS-7 cells, EBNA-LP was localized
predominantly in the cytoplasm and was colocalized with HAX-1. These
results indicate that EBNA-LP is in fact present and may have a
significant function in the cytoplasm, possibly by interacting with and
affecting the function of HAX-1.
*
Corresponding author. Mailing address: Department of
Tumor Virology, Division of Virology and Immunology, Medical Research Institute, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. Phone: 81-3-5803-5815. Fax: 81-3-5803-0241. E-mail: hirai.creg{at}mri.tmd.ac.jp.
Journal of Virology, November 2000, p. 10104-10111, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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