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Journal of Virology, November 2000, p. 10096-10103, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Infection of Dendritic Cells by the Maedi-Visna Lentivirus

Susanna Ryan,* Laurence Tiley, Ian McConnell, and Barbara Blacklaws

Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 OES, United Kingdom

Received 16 May 2000/Accepted 3 August 2000

The early stages of lentivirus infection of dendritic cells have been studied in an in vivo model. Maedi-visna virus (MVV) is a natural pathogen of sheep with a tropism for macrophages, but the infection of dendritic cells has not been proven, largely because of the difficulties of definitively distinguishing the two cell types. Afferent lymphatic dendritic cells from sheep have been phenotypically characterized and separated from macrophages. Dendritic cells purified from experimentally infected sheep have been demonstrated not only to carry infectious MVV but also to be hosts of the virus themselves. The results of the in vivo infection experiments are supported by infections of purified afferent lymph dendritic cells in vitro, in which late reverse transcriptase products are demonstrated by PCR. The significance of the infection of afferent lymph dendritic cells is discussed in relation to the initial spread of lentivirus infection and the requirement for CD4 T cells.

* Corresponding author. Mailing address: Wellcome Trust Immunology Unit, School of Clinical Medicine, Hills Road, Cambridge CB2 2SP, United Kingdom. Phone: 44 1223 330526. Fax: 44 1223 336815. E-mail: srr20{at}cam.ac.uk.

Journal of Virology, November 2000, p. 10096-10103, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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