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Journal of Virology, November 2000, p. 10025-10033, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Human Immunodeficiency Virus Prime-Boost Immunization Regimen in Humans Induces Antibodies That Show Interclade Cross-Reactivity and Neutralize Several X4-, R5-, and Dualtropic Clade B and C Primary Isolates

Florence Verrier,1 Sherri Burda,1 Robert Belshe,2 Anne-Marie Duliege,3 Jean-Louis Excler,4,dagger Michel Klein,4 and Susan Zolla-Pazner1,*

Veterans Affairs Medical Center and New York University School of Medicine, New York, New York1; St. Louis University, St. Louis, Missouri2; Chiron Corporation, Emeryville, California3; and Aventis Pasteur, Campus Merieux, Marcy L'Etoile, France4

Received 3 May 2000/Accepted 21 July 2000

A human immunodeficiency virus (HIV) vaccine that will be useful in diverse geographic regions will need to induce a broad immune response characterized by cross-clade immunity. To test whether a clade B-based HIV candidate vaccine could induce interclade humoral responses, including neutralizing activity against primary HIV-1 isolates, sera were tested from recipients of a vaccine consisting of recombinant canarypox virus vCP205 and recombinant gp120SF2. Serum antibodies exhibited strong immunochemical cross-reactivity with V3 peptides from clades B, C, and F, with weaker activity for several V3 peptides from clades A, D, G, and H; essentially no reactivity could be demonstrated with V3 peptides from clades E and O. Extensive cross-clade reactivity was also documented by enzyme-linked immunosorbent assay with all nine recombinant HIV envelope glycoproteins tested from clades B, D, and E. In addition, vaccinees' sera displayed significant neutralizing activity against 5 of 14 primary isolates tested, including one X4 virus and two dualtropic viruses (from clade B) and two R5 viruses (from clades B and C). This is the first demonstration of the induction by a candidate HIV vaccine constructed from clade B laboratory strains of HIV of neutralizing activity against R5 and clade C primary isolates. The data suggest that, by virtue of their ability to induce cross-clade immune responses, appropriately formulated HIV vaccines based on a finite number of HIV isolates may ultimately be able to protect against the wide range of HIV isolates affecting the populations of many geographic regions.

* Corresponding author. Mailing address: c/o VA Medical Center (Room 18124N), 423 E. 23rd St., New York, NY 10010. Phone: (212) 951-3211. Fax: (212) 951-6321. E-mail: zollas01{at}popmail.med.nyu.edu.

dagger Present address: Henry M. Jackson Foundation, Rockville, Md.

Journal of Virology, November 2000, p. 10025-10033, Vol. 74, No. 21
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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