Recombinant Adenovirus Induces Maturation of Dendritic Cells via an NF-kappa B-Dependent Pathway

doi:10.1128/JVI.74.20.9617-9628.2000

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Journal of Virology, October 2000, p. 9617-9628, Vol. 74, No. 20
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Recombinant Adenovirus Induces Maturation of Dendritic Cells via an NF- $kappa$ B-Dependent Pathway

Adrian E. Morelli,¹^,²^,^* Adriana T. Larregina,³ Raymond W. Ganster,² Alan F. Zahorchak,¹^,² Jeffrey M. Plowey,³ Takuya Takayama,¹^,² Alison J. Logar,¹^,² Paul D. Robbins,⁴ Louis D. Falo,³ and Angus W. Thomson¹^,²^,⁴

Thomas E. Starzl Transplantation Institute,¹ Department of Surgery,² Department of Molecular Genetics and Biochemistry,⁴ and Department of Dermatology and the University of Pittsburgh Cancer Institute,³ University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213-2582

Received 5 May 2000/Accepted 12 July 2000

Recombinant adenovirus (rAd) infection is one of the most effective and frequently employed methods to transduce dendriticcells (DC). Contradictory results have been reported recentlyconcerning the influence of rAd on the differentiation and activationof DC. In this report, we show that, as a result of rAd infection,mouse bone marrow-derived immature DC upregulate expression ofmajor histocompatibility complex class I and II antigens, costimulatorymolecules (CD40, CD80, and CD86), and the adhesion molecule CD54(ICAM-1). rAd-transduced DC exhibited increased allostimulatorycapacity and levels of interleukin-6 (IL-6), IL-12p40, IL-15,gamma interferon, and tumor necrosis factor alpha mRNAs, withouteffects on other immunoregulatory cytokine transcripts such asIL-10 or IL-12p35. These effects were not related to specifictransgenic sequences or to rAd genome transcription. The rAd effectcorrelated with a rapid increase (1 h) in the NF- $kappa$ B-DNA bindingactivity detected by electrophoretic mobility shift assays. rAd-inducedDC maturation was blocked by the proteasome inhibitor N $alpha$ -p-tosyl-L-lysinechloromethyl ketone (TLCK) or by infection with rAd-I $kappa$ B, an rAd-encodingthe dominant-negative form of I $kappa$ B. In vivo studies showed thatafter intravenous administration, rAds were rapidly entrappedin the spleen by marginal zone DC that mobilized to T-cell areas,a phenomenon suggesting that rAd also induced DC differentiationin vivo. These findings may explain the immunogenicity of rAdand the difficulties in inducing long-term antigen-specific T-cellhyporesponsiveness with rAd-transducedDC.

^* Corresponding author. Mailing address: Department of Surgery, University of Pittsburgh Medical Center, E1504 Biomedical ScienceTower, 200 Lothrop St., Pittsburgh, PA 15213. Phone: (412) 624-6627.Fax: (412) 624-1172. E-mail: morelli{at}imap.pitt.edu.

Journal of Virology, October 2000, p. 9617-9628, Vol. 74, No. 20
0022-538X/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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Recombinant Adenovirus Induces Maturation of Dendritic Cells via an NF-B-Dependent Pathway

Recombinant Adenovirus Induces Maturation of Dendritic Cells via an NF- $kappa$ B-Dependent Pathway